CELL DIVISION CONTROL PROTEIN48 (CDC48) is essential for membrane fusion, protein degrada-tion, and other cellular processes. Here, we re-vealed the crucial role of CDC48B in regulating periclinal cell division in roots by analyzing the recessive gen1 mutant. We identified the GEN1 gene through map-based cloning and verified that GEN1 encodes CDC48B. gen1 showed severely inhibited root growth, increased periclinal cell division in the endodermis, defective middle cortex (MC) formation, and altered ground tissue patterning in roots. Consistent with these pheno-types, CYCLIND 6;1(CYCD6;1), a periclinal cell di-vision marker, was upregulated in gen1 compared to Col-0. The ratio of SHRpro:SHR-GFP fluo-rescence in pre-dividing nuclei versus the ad-jacent stele decreased by 33% in gen1, indicating that the trafficking of SHORT-ROOT (SHR) de-creased in gen1 when endodermal cells started to divide. These findings suggest that the loss of function of CDC48B inhibits the intercellular traf-ficking of SHR from the stele to the endodermis, thereby decreasing SHR accumulation in the en-dodermis. These findings shed light on the crucial role of CDC48B in regulating periclinal cell division in roots.

Lihui Yang;Mingyue Zhu;Yi Yang;Ke Wang;Yulei Che;Shurui Yang;Jinxiang Wang;Xin Yu;Lixin Li;Shuang Wu;Klaus Palme;Xugang Li

State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Daizong Street 61,Tai'an 271018,China;Institute of Biology II/Molecular Plant Physiology,Faculty of Biology,Albert-Ludwigs-University of Freiburg,Sch?nzlestrasse 1,Freiburg D-79104,Germany;Department of Genetics,Northwest Women's and Children's Hospital,Xi'an 710061,China;Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land,Shandong Agricultural University,Daizong Street 61,Tai'an 271018,China;Sino German Joint Research Center for Agricultural Biology,College of Life Sciences,Shandong Agricultural University,Daizong Street 61,Tai'an 271018,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510640,China;Citrus Research Institute,Southwest University,Chongqing 400712,China;Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040,China;FAFU-UCR Joint Center and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China

植物学报（英文版）

[1]Lihui Yang;Mingyue Zhu;Yi Yang;Ke Wang;Yulei Che;Shurui Yang;Jinxiang Wang;Xin Yu;Lixin Li;Shuang Wu;Klaus Palme;Xugang Li-.CDC48B facilitates the intercellular trafficking of SHORT-ROOT during radial patterning in roots OO)[J].植物学报（英文版）,2022(04):843-858

CDC48B,DIVISION,PROTEIN48,CDC48,periclinal,gen1,GEN1,endodermis,CYCLIND,CYCD6,SHRpro,stele,endodermal,ficking,dodermis

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