典型文献
Smurf participates in Helicoverpa armigera diapause by regulating the transforming growth factor-β signaling pathway
文献摘要：
Diapause,an important strategy used by insects to avoid adverse environments,is regulated by various cell signaling pathways.The results of our previous studies demon-strated that the transforming growth factor-β(TGF-β)signaling pathway regulated pupal diapause in Helicoverpa armigera,which was accompanied by downregulation of pro-teins in TGF-β signaling.However,to date the mechanism underlying this phenomenon remains unknown.Here,we cloned the E3 ubiquitin ligases gene Smurf.In vitro experi-ments showed that Smurf directly bound to TGF-β receptor type I(TGFβRI)and Smad2.Overexpressing Smurf promoted ubiquitination of TGFβRI and Smad2,thereby down-regulating their protein levels.Conversely,silencing of the Smurf gene suppressed ubiq-uitination of TGFβRI and Smad2 thereby increasing their protein levels.Results from in vivo co-immunoprecipitation assays revealed that the binding of Smurf to TGFβRI or Smad2 was stronger in diapause pupae than in nondiapause pupae.Injection of Smurf in-hibitor A01 into diapause pupae markedly upregulated expression of TGFβRI and Smad2 proteins,leading to resumption of development in diapause pupae.Taken together,these findings suggested that ubiquitin ligase E3 Smurf participated in H.armigera diapause by regulating TGF-β signaling,and thus could be playing a crucial role in insect diapause.
文献关键词：
中图分类号：
作者姓名：
Haiyin Li;Qin Lu;Yan Li;Yufang Yan;Zhiyong Yin;Jianjun Guo;Weihua Xu
作者机构：
Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region,Institute of Entomology,Guizhou University,Guiyang,China;State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou,China
文献出处：
引用格式：
[1]Haiyin Li;Qin Lu;Yan Li;Yufang Yan;Zhiyong Yin;Jianjun Guo;Weihua Xu-.Smurf participates in Helicoverpa armigera diapause by regulating the transforming growth factor-β signaling pathway)[J].中国昆虫科学（英文版）,2022(05):1251-1261
A类：
Smurf,Diapause,ubiq,uitination,nondiapause
B类：
participates,Helicoverpa,armigera,regulating,transforming,growth,signaling,important,strategy,used,insects,avoid,adverse,environments,various,cell,pathways,results,our,previous,studies,demon,strated,that,TGF,pupal,which,was,accompanied,downregulation,However,date,mechanism,underlying,this,phenomenon,remains,unknown,Here,cloned,E3,ligases,gene,vitro,experi,showed,directly,bound,receptor,type,RI,Smad2,Overexpressing,promoted,ubiquitination,thereby,their,levels,Conversely,silencing,suppressed,increasing,Results,from,vivo,immunoprecipitation,assays,revealed,binding,stronger,pupae,than,Injection,hibitor,A01,into,markedly,upregulated,expression,proteins,leading,resumption,development,Taken,together,these,findings,suggested,participated,thus,could,be,playing,crucial,role
AB值：
0.464101
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
