典型文献
Single-cell RNA sequencing reveals the landscape of maize root tips and assists in identification of cell type-specific nitrate-response genes
文献摘要：
The root system is fundamental for maize growth and yield. Characterizing its heterogeneity and cell type-specific response to nitrate at the single-cell level will shed light on root development and nutrient uptake. We profiled the transcriptomes of>7000 cells derived from root tips of maize seedlings grown on media with or without nitrate, and identified 11 major cell types or tissues and 85 cell type-specific nitrate-response genes, including several known nitrate metabolic genes. A pseudotime analysis showed a continuous pseudotime series with the beginning at meristematic zone cells and showed that the root hair cell was derived by differentiation of a subset of epidermal cells. Interspecies comparison of root cells between maize and rice revealed the conservation and divergence of the root cell types and identified 57, 216, and 80 conserved orthologous genes in root hair, endodermis, and phloem cells respectively. This study provides a global view of maize root tip developmental processes and responses to nitrate at the single-cell level. The genes described in the present work could serve as targets for further genetic anal-yses and accurate regulation of gene expression and phenotypic variation in specific cell types or tissues.
文献关键词：
中图分类号：
作者姓名：
Xuhui Li;Xiangbo Zhang;Shuai Gao;Fangqing Cui;Weiwei Chen;Lina Fan;Yongwen Qi
作者机构：
Institute of Nanfan&Seed Industry,Guangdong Academy of Science,Guangzhou 510316,Guangdong,China;College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510325,Guangdong,China
文献出处：
引用格式：
[1]Xuhui Li;Xiangbo Zhang;Shuai Gao;Fangqing Cui;Weiwei Chen;Lina Fan;Yongwen Qi-.Single-cell RNA sequencing reveals the landscape of maize root tips and assists in identification of cell type-specific nitrate-response genes)[J].作物学报（英文版）,2022(06):1589-1600
A类：
pseudotime,meristematic,endodermis
B类：
Single,sequencing,reveals,landscape,maize,root,tips,assists,identification,specific,nitrate,genes,system,fundamental,growth,yield,Characterizing,its,heterogeneity,single,level,will,shed,light,nutrient,uptake,We,profiled,transcriptomes,cells,derived,from,seedlings,grown,media,without,identified,major,types,tissues,including,several,known,metabolic,analysis,showed,continuous,series,beginning,zone,that,hair,was,by,differentiation,subset,epidermal,Interspecies,comparison,between,rice,revealed,conservation,divergence,conserved,orthologous,phloem,respectively,This,study,provides,global,view,developmental,processes,responses,described,present,work,could,targets,further,genetic,yses,accurate,regulation,expression,phenotypic,variation
AB值：
0.509434
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