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典型文献
A G-type lectin receptor-like kinase regulates the perception of oomycete apoplastic expansin-like proteins
文献摘要:
Phytophthora capsici is one of the most harmful pathogens in agriculture,which threatens the safe production of multiple crops and causes serious economic losses worldwide.Here,we identified a P.capsici expansin-like protein,PcEXLX1,by liquid chromatography-tandem mass spectrometry from Nicotiana benthamiana apoplastic fluid infected with P.capsici.Clustered regularly interspaced short palindromic repeats/crispr associated protein 9 (CRISPR/Cas9)-mediated PcEXLX1 knockout mutants exhibited significantly enhanced virulence,while the overexpression of PcEXLX1 impaired the virulence.Prokaryotically expressed PcEXLX1 acti-vated multiple plant immune responses,which were BRI1-associated kinase 1 (BAK1)-and suppressor of BIR1-1 (SOBIR1)-dependent.Furthermore,over-expression of PcEXLX1 homologs in N.ben-thamiana could also increase plant resistance to P.capsici.A G-type lectin receptor-like kinase from N.benthamiana,expansin-regulating kinase 1(ERK1),was shown to regulate the perception of PcEXLX1 and positively mediate the plant resist-ance to P.capsici.These results reveal that the expansin-like protein,PcEXLX1,is a novel apoplastic effector with plant immunity-inducing activity of oomycetes,perception of which is regu-lated by the receptor-like kinase,ERK1.
文献关键词:
作者姓名:
Lei Pi;Zhiyuan Yin;Weiwei Duan;Nan Wang;Yifan Zhang;Jinghao Wang;Daolong Dou
作者机构:
College of Plant Protection,China Agricultural University,Beijing 100193,China;College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China
引用格式:
[1]Lei Pi;Zhiyuan Yin;Weiwei Duan;Nan Wang;Yifan Zhang;Jinghao Wang;Daolong Dou-.A G-type lectin receptor-like kinase regulates the perception of oomycete apoplastic expansin-like proteins)[J].植物学报(英文版),2022(01):183-201
A类:
PcEXLX1,crispr,Prokaryotically,BIR1,SOBIR1,thamiana,oomycetes
B类:
type,lectin,receptor,like,kinase,regulates,perception,apoplastic,expansin,proteins,Phytophthora,capsici,one,most,harmful,pathogens,agriculture,which,threatens,safe,production,multiple,crops,causes,serious,economic,losses,worldwide,Here,identified,by,liquid,chromatography,tandem,mass,spectrometry,from,Nicotiana,benthamiana,fluid,infected,Clustered,regularly,interspaced,short,palindromic,repeats,associated,CRISPR,Cas9,mediated,knockout,mutants,exhibited,significantly,enhanced,virulence,while,overexpression,impaired,expressed,vated,plant,immune,responses,were,BRI1,BAK1,suppressor,dependent,Furthermore,homologs,could,also,increase,resistance,regulating,ERK1,was,shown,positively,These,results,reveal,that,novel,effector,immunity,inducing,activity,lated
AB值:
0.50033
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