Ionotropic receptors(IRs)were first found in Drosophila melanogaster,and derive from ionotropic glutamate receptors(iGluRs),which are implicated in detecting acids,ammonia,amine,temperature and humidity.Although IRs are involved in sens-ing acid odors in a few insects,such as D.melanogaster,Aedes aegypti,and Manduca sexta,the function of IRs in Helicoverpa armigera is still unknown.IR8a was confirmed to be a co-receptor associated with acid detection.From the results of phylogenetic anal-ysis,HarmIR8a displayed high similarity compared to homologs in D.melanogaster,M.sexta,and A.aegypti,suggesting that HarmIR8a might have a consistent function as a co-receptor for acid detection.In this study,clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated genome editing was implemented to knockout HarmIR8a for in vivo functional analysis.Electrophysiologi-cal and behavioral assays were performed to compare the differences between HarmIR8a knockout mutants and wild type individuals.From electroantennogram(EAG)analysis,we found that wild type H.armigera adults could detect short-chain carboxylic acids.In addi-tion,wind tunnel experiments showed that 1％acetic acid attracted wild type H.armigera adults.However,acid sensing and attraction were reduced or abolished in the HarmIR8a knockout mutants.Our data suggest that HarmIR8a is important for H.armigera to detect short-chain carboxylic acids and mediate attraction behavior to acetic acid.

Xia-Xuan Zhang;Bin Yang;Dong-Dong Sun;Meng-Bo Guo;Jie Zhang;Gui-Rong Wang

State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sci-ences,Beijing,China;Lingnan Guangdong Laboratory of Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong Province,China;College of Life Sciences,Anhui Normal University,Wuhu,Anhui Province,China

中国昆虫科学（英文版）

[1]Xia-Xuan Zhang;Bin Yang;Dong-Dong Sun;Meng-Bo Guo;Jie Zhang;Gui-Rong Wang-.Ionotropic receptor 8a is involved in the attraction of Helicoverpa armigera to acetic acid)[J].中国昆虫科学（英文版）,2022(03):657-668

iGluRs,odors,IR8a,HarmIR8a,Electrophysiologi

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