Heading date (or flowering time), an important agronomic trait in crop species, is closely associated with regional adaptation and yield. Members of the Pseudo-Response Regulator (PRR) family play key roles in regulating flowering. However, their role and molecular mechanism controlling heading date in rice is not very clear. Here, we identified rice OsPRR protein, OsPRR59, which delayed heading under long-day conditions. OsPRR59 positively regulates yield by affecting plant height, secondary branches number per panicle, grain number per panicle, seed setting rate, and grain weight per plant. OsPRR59 is expressed in most tissues and its protein is localized to the nucleus. We also found that OsPRR59 directly binds to the promoter of Ehd3 to inhibit its expression. Compared with the WT, osprr59 ehd3 showed a signifi-cantly delayed heading phenotype, as did the ehd3 mutant. This was opposite to the phenotype of the osprr59 mutant, confirming that Ehd3 acted downstream of OsPRR59 in regulating rice flowering. Our results identified a direct regulator of Ehd3, and revealed a novel molecular mechanism of clock compo-nent OsPRR proteins in regulating heading date and provide a new genetic resource for fine-tuning head-ing date in rice.

Yupeng Wang;Fuqing Wu;Shirong Zhou;Weiwei Chen;Chenyan Li;Erchao Duan;Jiachang Wang;Zhijun Cheng;Xin Zhang;Qibing Lin;Yulong Ren;Cailin Lei;Xiuping Guo;Ziming Wu;Shanshan Zhu;Jianmin Wan

National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;National Key Laboratory for Crop Genetics and Germplasm Enhancement,Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China

作物学报（英文版）

[1]Yupeng Wang;Fuqing Wu;Shirong Zhou;Weiwei Chen;Chenyan Li;Erchao Duan;Jiachang Wang;Zhijun Cheng;Xin Zhang;Qibing Lin;Yulong Ren;Cailin Lei;Xiuping Guo;Ziming Wu;Shanshan Zhu;Jianmin Wan-.Clock component OsPRR59 delays heading date by repressing transcription of Ehd3 in rice)[J].作物学报（英文版）,2022(06):1570-1579

OsPRR59,Ehd3,OsPRR,osprr59,ehd3

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