典型文献
Optical tissue clearing enables rapid,precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
文献摘要：
Morphological analyses are key outcome assessments for nerve regeneration studies but are historically limited to tissue sections.Novel optical tissue clearing techniques enabling three-dimensional imaging of entire organs at a subcellular resolution have revolutionized morphological studies of the brain.To extend their applicability to experimental nerve repair studies we adapted these techniques to nerves and their motor and sensory targets in rats.The solvent-based protocols rendered harvested peripheral nerves and their target organs transparent within 24 hours while preserving tissue architecture and fluorescence.The optical clearing was compatible with conventional laboratory techniques,including retrograde labeling studies,and computational image segmentation,providing fast and precise cell quantitation.Further,optically cleared organs enabled three-dimensional morphometry at an unprecedented scale including dermatome-wide innervation studies,tracing of intramuscular nerve branches or mapping of neurovascular networks.Given their wide-ranging applicability,rapid processing times,and low costs,tissue clearing techniques are likely to be a key technology for next-generation nerve repair studies.All procedures were approved by the Hospital for Sick Children's Laboratory Animal Services Committee(49871/9)on November 9,2019.
文献关键词：
中图分类号：
作者姓名：
Simeon C.Daeschler;Jennifer Zhang;Tessa Gordon;Gregory H.Borschel
作者机构：
SickKids Research Institute,Neuroscience and Mental Health Program,Toronto,ON,Canada;Division of Plastic and Reconstructive Surgery,the Hospital for Sick Children,Toronto,ON,Canada;Institute of Biomaterials and Biomedical Engineering,University of Toronto,Toronto,ON,Canada;Indiana University School of Medicine,Indianapolis,IN,USA
文献出处：
引用格式：
[1]Simeon C.Daeschler;Jennifer Zhang;Tessa Gordon;Gregory H.Borschel-.Optical tissue clearing enables rapid,precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research)[J].中国神经再生研究（英文版）,2022(06):1348-1356
A类：
dermatome
B类：
Optical,tissue,clearing,enables,rapid,precise,comprehensive,three,dimensional,morphology,experimental,regeneration,research,Morphological,analyses,key,outcome,assessments,studies,but,historically,limited,sections,Novel,techniques,enabling,imaging,entire,organs,subcellular,resolution,have,revolutionized,morphological,brain,To,extend,their,applicability,repair,adapted,these,nerves,motor,sensory,targets,rats,solvent,protocols,rendered,harvested,peripheral,transparent,within,hours,while,preserving,architecture,fluorescence,was,compatible,conventional,laboratory,including,retrograde,labeling,computational,image,segmentation,providing,fast,quantitation,Further,optically,cleared,enabled,morphometry,unprecedented,scale,wide,innervation,tracing,intramuscular,branches,mapping,neurovascular,networks,Given,ranging,processing,times,low,costs,likely,technology,next,All,procedures,were,approved,by,Hospital,Sick,Children,Laboratory,Animal,Services,Committee,November
AB值：
0.645874
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
