OBJECTIVE:To explore the novel biomarkers and therapeutic target candidates related to the stasis-heat syndrome of acute intracerebral hemorrhage(AICH).METHODS:Applying an isobaric tagging for relative and absolute quantitation-(iTRAQ-)based quantitative proteomic approach,plasma samples from AICH patients with stasis-heat,and AICH patients with non-stasis-heat and healthy control subjects were collected and analyzed to distinguish differentially expressed proteins(DEPs)correlated to AICH with stasis-heat in this block design.The standard Western blot was applied to verify DEPs.Additionally,DEPs were analyzed via bioinformatic platforms and further approved via Ingenuity Pathway Analysis(IPA).RESULTS:A total of 26 DEPs were found among AICH with the stasis-heat,AICH with non-stasis-heat,and healthy control group.The seven DEPs compared with the non-stasis-heat group are closely related to the pathogenesis of stasis heat.These proteins showed three different protein expression patterns.The alpha-1-b glycoprotein(A1BG)and copper-protein(CP)were up-regulated in the stasis-heat group,but down-regulated in the non-stasis-heat group.Compared with the non-stasis-heat group,the expression abundance of actinin,alpha 1(ACTN1),carbonic anhydrase I(CA1),peroxiredoxin 2(PRDX2),and vinculin(VCL)is higher in the stasis-heat group,while the CD44 is the opposite.These differences reflect that stasis-heat syndrome has more severe inflammatory immune response,coagulation disorders and damage.Bioinformatics analysis revealed that a wide variety of cellular and metabolic processes and some signaling pathways were involved in the pathophysiology of AICH with stasis-heat.AICH with stasis-heat syndrome showed more severe inflammatory reactions,tissue damage,and coagulation disorders than non-stasis heat syndrome.CONCLUSIONS:There are differences in the protein expression patterns between the stasis-heat syndrome and non-stasis-heat syndrome.These differences reflect that stasis-heat syndrome has more severe damage.CD44,CP,ACTN1,CA1,VCL,PRDX2,and A1BG could be the potential biomarkers and therapeutic target candidates of the stasis-heat subtype.This study provides a reasonable explaination for Liangxue Tongyu decoction through anti-inflammatory and brain protection treatment.

WEI Lexin;LI Weiyi;TIAN Ting;ZHANG Ning;YANG Shijing;YANG Dongqing;LI Guochun;YE Fang

Department of Public Health,School of Medicine and Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China;Emergency Department,Nanjing Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine,Nanjing 210001,China;the Key Laboratory Department of Stasis-heat Pathogenesis of Traditional Chinese Medicine,the First Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210001,China

中医杂志（英文版）

[1]WEI Lexin;LI Weiyi;TIAN Ting;ZHANG Ning;YANG Shijing;YANG Dongqing;LI Guochun;YE Fang-.Identification of novel biomarkers and therapeutic target candidates for stasis-heat symptom pattern of acute intracerebral hemorrhage by quantitative plasma proteomics)[J].中医杂志（英文版）,2022(04):622-632

A1BG,explaination,Liangxue,Tongyu

Identification,novel,biomarkers,therapeutic,target,candidates,stasis,heat,symptom,acute,intracerebral,hemorrhage,by,quantitative,plasma,proteomics,OBJECTIVE,explore,syndrome,AICH,METHODS,Applying,isobaric,tagging,relative,absolute,quantitation,iTRAQ,approach,samples,from,patients,healthy,control,subjects,were,collected,analyzed,distinguish,differentially,expressed,proteins,DEPs,correlated,this,block,design,standard,blot,was,applied,verify,Additionally,via,bioinformatic,platforms,further,approved,Ingenuity,Pathway,Analysis,IPA,RESULTS,total,found,among,group,seven,compared,closely,pathogenesis,These,showed,three,expression,patterns,alpha,glycoprotein,copper,CP,regulated,but,down,Compared,abundance,actinin,ACTN1,carbonic,anhydrase,CA1,peroxiredoxin,PRDX2,vinculin,VCL,higher,while,CD44,opposite,differences,reflect,that,has,more,severe,inflammatory,immune,response,coagulation,disorders,damage,Bioinformatics,analysis,revealed,wide,variety,cellular,metabolic,processes,some,signaling,pathways,involved,pathophysiology,reactions,tissue,than,CONCLUSIONS,There,between,could,potential,subtype,This,study,provides,reasonable,decoction,through,brain,protection,treatment

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