Herein,the nanoscaled ATP-responsive upconversion metal-organic frameworks (UCMOFs) are aqueous-phase synthesized for co-delivery of therapeutic protein cytochrome c (Cyt c) and chemodrugs doxoru-bicin (DOX),achieving targeted combinational therapy of human cervical cancer.The UCMOFs are ra-tionally fabricated by growing ZIF-90 on mesoporous silica-coated upconversion nanoparticles (UCNPs),in which the ZIF-90 layer attenuates the upconversion luminescence (UCL) and the rigid frameworks in-crease the stability of encapsulated proteins.Once the UCMOF@DOX/Cyt c are internalized into HeLa cells via specific recognition of sgc8 aptamers,the intracellular ATP triggers the dissolution of ZIF-90 into Zn2+,which facilitates not only the release of Cyt c and DOX but also the restoration of UCL for real-time mon-itoring of drug release.It has been demonstrated that the therapeutic efficacy is greatly improved by the combination of caspase-mediated apoptosis activated by Cyt c (protein therapeutics),DNA fragmentation induced by DOX (chemotherapy),and Zn2+-promoted generation of reactive oxygen species (ROS) (ox-idative stress).Overall,our proposed multifunctional UCMOFs provide an effective platform for targeted combinational cancer therapy and in situ imaging,which hold great promise in biomedical and clinical applications.

Lin Yang;Shuaidong Zhu;Zhimei He;Xiangli Li;Jiangning Chen;Sai Bi;Jun-Jie Zhu

State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,School of Life Sciences,Nanjing University,Nanjing 210023,China;College of Chemistry and Chemical Engineering,Qingdao University,Qingdao 266071,China

中国化学快报（英文版）

[1]Lin Yang;Shuaidong Zhu;Zhimei He;Xiangli Li;Jiangning Chen;Sai Bi;Jun-Jie Zhu-.Aqueous-phase synthesis of upconversion metal-organic frameworks for ATP-responsive in situ imaging and targeted combinational cancer therapy)[J].中国化学快报（英文版）,2022(01):314-319

nanoscaled,UCMOFs,chemodrugs,doxoru,UCMOF,sgc8,idative

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