Choriogenesis is the last step of insect oogenesis, a process by which the chorion polypeptides are produced by the follicular cells and deposited on the surface of oocytes in order to provide a highly specialized protective barrier to the embryo. The essential features of chorion genes have yet to be clearly understood in the diamondback moth, Plutella xylostella, a worldwide Lepidoptera pest attacking cruciferous crops and wild plants. In this study, complete sequences for 15 putative chorion genes were identified, and grouped into A and B classes. Phylogenetic analysis revealed that both classes were highly conserved and within each, branches are also species-specific. Chorion genes from each class were located in pairs on scaffolds of the P. xylostella genome, some of which shared the common promoter regulatory region. All chorion genes were highly specifically expressed in the P. xylostella adult females, mostly in the ovary with full yolk, which is a crucial period to build the shells of the eggs. RNAi-based knockdown of chorion-1, which is located on the Px_scaffold 6 alone, although had no effect on yolk deposition, resulted in smaller eggs and sharply reduced hatchability. Additionally, inhibition of PxCho-1 expression caused a less dense arrangement of the columnar layers, reduced exochorion roughness and shorter microvilli. Our study provides the foundation for exploring molecular mechanisms of female reproduction in P. xylostella, and for making use of chorion genes as the potential genetic-based molecular target to better control this economically important pest.

DONG Shi-jie;LIU Bo;ZOU Ming-min;LIU Li-li;CAO Min-hui;HUANG Meng-qi;LIU Yan;Liette VASSEUR;YOU Min-sheng;PENG Lu

State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops,Institute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China;International Joint Research Laboratory of Ecological Pest Control of Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China;Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China;Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops,Ministry of Agriculture and Rural Affairs,Fuzhou 350002,P.R.China;Fujian Provincial Key Laboratory of Insect Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,P.R.China;Department of Biological Sciences,Brock University,St.Catharines,Ontario L2S 3A1,Canada

农业科学学报（英文）

[1]DONG Shi-jie;LIU Bo;ZOU Ming-min;LIU Li-li;CAO Min-hui;HUANG Meng-qi;LIU Yan;Liette VASSEUR;YOU Min-sheng;PENG Lu-.Identification of chorion genes and RNA interference-mediated functional characterization of chorion-1 in Plutella xylostella)[J].农业科学学报（英文）,2022(11):3278-3292

Choriogenesis,oogenesis,Chorion,hatchability,PxCho,exochorion,microvilli

Identification,interference,mediated,functional,characterization,Plutella,xylostella,last,step,insect,process,by,which,polypeptides,produced,follicular,cells,deposited,surface,oocytes,order,highly,specialized,protective,barrier,embryo,essential,features,have,yet,clearly,understood,diamondback,moth,worldwide,Lepidoptera,pest,attacking,cruciferous,crops,wild,plants,In,this,study,complete,sequences,putative,were,identified,grouped,into,classes,Phylogenetic,analysis,revealed,that,both,conserved,within,each,branches,also,species,from,located,pairs,scaffolds,genome,some,shared,common,promoter,regulatory,region,All,specifically,expressed,adult,females,mostly,ovary,full,yolk,crucial,period,build,shells,eggs,RNAi,knockdown,alone,although,had,effect,deposition,resulted,smaller,sharply,reduced,Additionally,inhibition,expression,caused,less,dense,arrangement,columnar,layers,roughness,shorter,Our,provides,foundation,exploring,molecular,mechanisms,reproduction,making,potential,target,better,control,economically,important

0.559076