Many holo-and hemimetabolous insects enhance their eggshells during embryogenesis by forming a serosal cuticle(SC).To date,scholarly understanding of the SC composition and SC-related gene functions has been limited,especially for hemimetabolous insects.In this study,we initially performed transmission electron mi-croscopic(TEM)observation and chitin staining of the SC in Nilaparvata lugens,a hemimetabolous rice pest known as the brown planthopper(BPH).We confirmed that the SC was a chitin-rich lamellar structure deposited gradually during the early embryo-genesis.Parental RNA interference(RNAi)against Nilaparvata lugens chitin synthase 1(NlCHS1)in newly emerged and matured females resulted in decreases of egg hatchabil-ity by 100％and 76％,respectively.Ultrastructural analyses revealed loss of the lamellar structure of the SC in dsNlCHS1-treated eggs.According to temporal expression pro-files,five cuticle protein coding genes,NlugCpr1/2/3/8/90,were specifically or highly expressed during the SC formation period,and NlugCpr1/2/3/90 were further detected in 72 h eggshells extract by ultra-performance liquid chromatography-tandem mass spec-trometry/mass spectrometry.NlugCpr2/3/90 were likely three SC-specific cuticle proteins.TEM observations of the SC following parental RNAi against NlugCpr1/2/3/8/90 demon-strated that NlugCpr3/8/90 were essential for SC formation.The study provided an under-standing of the SC formation process and SC-related cuticle proteins in BPHs,which offer potential targets for pest control in the egg stage as well.

Jia-Bao Lu;Jian-Sheng Guo;Xuan Chen;Chen Cheng;Xu-Mei Luo;Xiao-Ya Zhang;Bernard Moussian;Jian-Ping Chen;Jun-Min Li;Chuan-Xi Zhang

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Key Laboratory of Biotechnol-ogy in Plant Protection of MOA of China and Zhejiang Province,Institute of Plant Virology,Ningbo University,Ningbo,Zhejiang Province,China;Institute of Insect Science,Zhejiang University,Hangzhou,China;School of Medicine,Zhejiang University,Hangzhou,China;Université C?te d'Azur,CNRS,Inserm,Institute of Biology Valrose,Nice,France

中国昆虫科学（英文版）

[1]Jia-Bao Lu;Jian-Sheng Guo;Xuan Chen;Chen Cheng;Xu-Mei Luo;Xiao-Ya Zhang;Bernard Moussian;Jian-Ping Chen;Jun-Min Li;Chuan-Xi Zhang-.Chitin synthase 1 and five cuticle protein genes are involved in serosal cuticle formation during early embryogenesis to enhance eggshells in Nilaparvata lugens)[J].中国昆虫科学（英文版）,2022(02):363-378

serosal,eggshells,hemimetabolous,NlCHS1,hatchabil,dsNlCHS1,NlugCpr1,NlugCpr2,NlugCpr3,BPHs

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