典型文献
A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant GmSHMT08
文献摘要：
Soybean cyst nematode (SCN) Heterodera glycines is considered as the major constraint to soybean production. GmSHMT08 at Rhg4 locus on chromosome 08, encoding a serine hydroxylmethyltransferase, is a major gene underlying resistance against H. glycines in Peking-type soybeans. However, the molecular mechanism underpinning this resistance is less well characterized, and whether GmSHMT08 could interact with proteins in H. glycines remains unclear. In this study, yeast two-hybrid screening was conducted using GmSHMT08 as a bait protein, and a fragment of a 70-kDa heat shock protein (HgHSP70) was screened from H. glycines that exhibited interaction with GmSHMT08. This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays. Our finding reveals HgHSP70 could be applied as a potential candidate gene for further exploring the mechanism on GmSHMT08-mediated resistance against SCN H. glycines.
文献关键词：
中图分类号：
作者姓名：
LIU Zhi;ZHANG Liu-ping;ZHAO Jie;JIAN Jin-zhuo;PENG Huan;HUANG Wen-kun;KONG Ling-an;PENG De-liang;LIU Shi-ming
作者机构：
State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China;MARA–CABI Joint Laboratory for Bio-safety,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China
文献出处：
引用格式：
[1]LIU Zhi;ZHANG Liu-ping;ZHAO Jie;JIAN Jin-zhuo;PENG Huan;HUANG Wen-kun;KONG Ling-an;PENG De-liang;LIU Shi-ming-.A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant GmSHMT08)[J].农业科学学报（英文）,2022(10):2973-2983
A类：
HgHSP70,GmSHMT08,Rhg4,hydroxylmethyltransferase
B类：
fragment,kDa,Heterodera,glycines,heat,shock,interacts,cyst,nematode,resistant,Soybean,SCN,considered,major,constraint,production,locus,chromosome,encoding,serine,gene,underlying,resistance,against,Peking,type,soybeans,However,mechanism,underpinning,this,less,well,characterized,whether,could,proteins,remains,unclear,In,study,yeast,two,hybrid,screening,was,conducted,using,bait,screened,from,that,exhibited,interaction,This,verified,by,both,GST,pull,down,bimolecular,fluorescence,complementation,assays,Our,finding,reveals,applied,potential,candidate,further,exploring,mediated
AB值：
0.517827
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