典型文献
Study on burrowing nematode, Radopholus similis, pathogenicity test system in tobacco as host
文献摘要：
Radopholus similis (Cobb 1893) Thorne (1949) is a destructive migratory endoparasitic plant nematode. In this study, the pathogenic process of R. similis infection in Nicotiana benthamiana (tobacco) was studied using quartz sand culture in laboratory. The results showed that R. similis mainly parasitised the root cortex, leading to cortical cell decomposition and tissue decay. We optimised the inoculation conditions to establish a method for determining the pathogenicity of R. similis as follows: (1) a glass culture tube was filled with quartz sand (about 1/3 of the height) and sterilised twice; (2) 20-day-old N. benthamiana seedlings were transplanted into test tubes and cultivated for 10 days at (25±1)℃; (3) R. similis female nematodes were inoculated in the root rhizosphere at a rate of 150 nematodes per plant; (4) the number of nematodes, disease severity, and growth of the plant at 30 days post-inoculation (dpi) were determined. The pathogenicity of eight R. similis populations from different hosts was determined, which proved the feasibility of this method.
文献关键词：
中图分类号：
作者姓名：
YANG Si-hua;ZHAO Li-rong;DING Sha;TANG Shi-qiao;CHEN Chun;ZHANG Huan-xin;XUChun-ling;XIE Hui
作者机构：
Research Center of Nematodes of Plant Quarantine,Laboratory of Plant Nematology,College of Plant Protection,South China Agricultural University,Guangzhou 510642,P.R.China;Technology Center,Guangzhou Customs District,People's Republic of China,Guangzhou 510623,P.R.China;Comprehensive Technology and Service Center of Huizhou Port Customs,Huizhou 516080,P.R.China
文献出处：
引用格式：
[1]YANG Si-hua;ZHAO Li-rong;DING Sha;TANG Shi-qiao;CHEN Chun;ZHANG Huan-xin;XUChun-ling;XIE Hui-.Study on burrowing nematode, Radopholus similis, pathogenicity test system in tobacco as host)[J].农业科学学报（英文）,2022(09):2652-2664
A类：
burrowing,Radopholus,endoparasitic,parasitised,sterilised
B类：
Study,similis,pathogenicity,test,system,tobacco,Cobb,Thorne,destructive,migratory,In,this,study,process,infection,Nicotiana,benthamiana,was,studied,using,quartz,sand,culture,laboratory,results,showed,that,mainly,root,cortex,leading,cortical,cell,decomposition,tissue,decay,We,optimised,inoculation,conditions,establish,method,determining,follows,glass,filled,about,height,twice,old,seedlings,were,transplanted,into,tubes,cultivated,days,female,nematodes,inoculated,rhizosphere,rate,per,number,disease,severity,growth,post,dpi,determined,populations,from,different,hosts,which,proved,feasibility
AB值：
0.516975
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