Pea aphid, Acyrthosiphon pisum, is a serious pest of many different leguminous plants, and it mainly relies on its odorant receptors (Ors) to discriminate among host species. However, less is known about the role that Ors play in the host plant location. In this study, we identified a novel conserved odorant receptor clade by phylogenetic analysis, and conducted the functional analysis of ApisOr23 in A. pisum. The results showed that the homologous Ors from A. pisum, Aphis glycines and Aphis gossypii share 94.28％ identity in amino acid sequences. Moreover, conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species, providing further proof of the high conservation level of the Or23 clade. According to the tissue expression pattern analysis, ApisOr23 was mainly expressed in the antennae. Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles, namely trans-2-hexen-1-al, cis-2-hexen-1-ol, 1-heptanol, 4′-ethylacetophenone, and hexyl acetate. Among them, trans-2-hexen-1-al, which is one of the main volatile organic compounds released from legume plants, activated the highest response of ApisOr23. Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.

HUANG Tian-yu;ZHANG Rui-bin;YANG Lu-lu;CAO Song;Frederic FRANCIS;WANG Bing;WANG Gui-rong

State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China;Functional and Evolutionary Entomology,Gembloux Agro-Bio Tech,University of Liège,Gembloux 5030,Belgium;Guangdong Laboratory for Lingnan Modern Agriculture(Shenzhen Branch),Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,P.R.China

农业科学学报（英文）

[1]HUANG Tian-yu;ZHANG Rui-bin;YANG Lu-lu;CAO Song;Frederic FRANCIS;WANG Bing;WANG Gui-rong-.Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum)[J].农业科学学报（英文）,2022(05):1414-1423

ApisOr23,Ors,Or23,hexen,heptanol,ethylacetophenone

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