典型文献
Identification of reference genes provides functional insights into meiotic recombination suppressors in Gerbera hybrida
文献摘要：
Gerbera Hybrida is one of the important cut flowers across the world. The novel traits are the primarily market requirements and the breeding targets, mainly determined by the degree of genetic variation after hybridization. However, meiotic recombination is highly conserved in most eukaryotes which suppressed the crossover formation and limited the genetic diversity. Recently, several meiotic recombination suppressors have been identified and characterized in plants, whereas it remains elusive in G. hybrida. In order to characterize the expression patterns of these suppressors in G. hybrida, 20 candidate reference genes were identified from the transcriptome datasets of G. hybrida, and their expression stabilities during plant development were evaluated by geNorm, NormFinder and BestKeeper. Although the most stable reference genes were variable in different softwares, comprehensive ranking revealed that PGK2 was the most stable reference gene and GAPDH was the most un-stable one. The expression patterns of FANCM, FIGL1, RECQ4, RM1, and FLIP further validated that PGK2 was suitable for normalization of gene expression. Our study identified a reliable reference gene for gene expression during meiotic recombination, and provided functional insights into meiotic recombination suppressors in G. hybrida.
文献关键词：
中图分类号：
作者姓名：
Fan Li;Ying Cheng;Lulin Ma;Shenchong Li;Jihua Wang
作者机构：
Floriculture Research Institute,Yunnan Academy of Agricultural Sciences,National Engineering Research Center for Ornamental Horticulture,Key Laboratory for Flower Breeding of Yunnan Province,Kunming 650200,China;School of Agriculture,Yunnan University,Kunming 650504,China
文献出处：
引用格式：
[1]Fan Li;Ying Cheng;Lulin Ma;Shenchong Li;Jihua Wang-.Identification of reference genes provides functional insights into meiotic recombination suppressors in Gerbera hybrida)[J].园艺学报（英文版）,2022(01):123-132
A类：
Hybrida,softwares,PGK2,FANCM,FIGL1,RECQ4
B类：
Identification,reference,genes,provides,functional,insights,into,meiotic,recombination,suppressors,Gerbera,hybrida,is,one,important,cut,flowers,across,world,novel,traits,primarily,market,requirements,breeding,targets,mainly,determined,by,degree,genetic,variation,after,hybridization,However,highly,conserved,most,eukaryotes,which,suppressed,crossover,formation,limited,diversity,Recently,several,have,been,identified,characterized,plants,whereas,remains,elusive,In,order,expression,patterns,these,candidate,were,from,transcriptome,datasets,their,stabilities,during,development,evaluated,geNorm,NormFinder,BestKeeper,Although,stable,variable,different,comprehensive,ranking,revealed,that,was,GAPDH,RM1,FLIP,further,validated,suitable,normalization,Our,study,reliable,provided
AB值：
0.510868
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
