典型文献
P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein
文献摘要：
Although the complete nucleotide sequence of strawberry vein banding virus (SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV (SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast two-hybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus.
文献关键词：
中图分类号：
作者姓名：
RUI Peng-huan;WANG Zhan-qi;SHAN Wen-shu;XIA Wei-wei;ZHOU Xiu-hong;YANG Lian-lian;JIANG Lei;JIANG Tong
作者机构：
School of Plant Protection,Anhui Agricultural University,Hefei 230036,P.R.China;Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province,College of Life Sciences,Huzhou University,Huzhou 313000,P.R.China;Biotechnology Center,Anhui Agricultural University,Hefei 230036,P.R.China;Anhui Province Key Laboratory of Integrated Pest Management on Crops,Hefei 230036,P.R.China;Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes,Anhui Agricultural University,Hefei 230036,P.R.China
文献出处：
引用格式：
[1]RUI Peng-huan;WANG Zhan-qi;SHAN Wen-shu;XIA Wei-wei;ZHOU Xiu-hong;YANG Lian-lian;JIANG Lei;JIANG Tong-.P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein)[J].农业科学学报（英文）,2022(04):1071-1083
A类：
multilocalized,SVBV,Caulimovirus
B类：
P1,strawberry,vein,banding,functions,movement,interacts,coat,Although,complete,nucleotide,sequence,has,been,determined,bioinformatic,analysis,revealed,that,genome,could,encode,seven,proteins,precise,each,unclear,This,study,provided,evidence,possesses,following,features,Bioinformatic,subcellular,localization,analyses,showed,cytoplasm,walls,epidermal,cells,Nicotiana,benthamiana,forms,inclusion,bodies,associated,microtubules,endoplasmic,reticulum,Dilution,experiments,demonstrated,from,original,agro,infiltrated,adjacent,leaves,Further,trans,complementation,facilitate,intercellular,deficient,potato,mutant,Finally,yeast,two,hybrid,bimolecular,fluorescence,assays,which,major,component,virions,Results,electrophoretic,mobility,shift,indicated,lacks,binding,capability,In,summary,results,suggest,probably,providing,new,insights,into,genus
AB值：
0.508385
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