典型文献
Coat protein of Chinese wheat mosaic virus upregulates and interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase, a negative regulator of plant autophagy, to promote virus infection
文献摘要：
Autophagy is an intracellular degradation mechanism involved in antiviral defense, but the strategies employed by plant viruses to counteract autophagy-related defense remain unknown for the majority of the viruses. Herein, we describe how the Chinese wheat mosaic virus (CWMV, genus Furovirus) interferes with autophagy and enhances its infection in Nicotiana benthamiana. Yeast two-hybrid screening and in vivo/in vitro assays revealed that the 19 kDa coat protein (CP19K) of CWMV interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs), negative regulators of autophagy, which bind auto-phagy-related protein 3 (ATG3), a key factor in autophagy. CP19K also directly interacts with ATG3, possibly leading to the formation of a CP19K–GAPC–ATG3 complex. CP19K–GAPC interaction appeared to intensify CP19K–ATG3 binding. Moreover, CP19K expression upregu-lated GAPC gene transcripts and reduced au-tophagic activities. Accordingly, the silencing of GAPC genes in transgenic N. benthamiana reduced CWMV accumulation, whereas CP19K overexpression enhanced it. Overall, our results suggest that CWMV CP19K interferes with au-tophagy through the promotion and utilization of the GAPC role as a negative regulator of autophagy.
文献关键词：
中图分类号：
作者姓名：
Erbo Niu;Chaozheng Ye;Wanying Zhao;Hideki Kondo;Yunfeng Wu;Jianping Chen;Ida Bagus Andika;Liying Sun
作者机构：
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection,Northwest A&F University,Xi'an 712100,China;Institute of Plant Science and Resources,Okayama University,Kurashiki 710-0046,Japan;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Plant Virology,Ningbo University,Ningbo 315211,China;College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,China
文献出处：
引用格式：
[1]Erbo Niu;Chaozheng Ye;Wanying Zhao;Hideki Kondo;Yunfeng Wu;Jianping Chen;Ida Bagus Andika;Liying Sun-.Coat protein of Chinese wheat mosaic virus upregulates and interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase, a negative regulator of plant autophagy, to promote virus infection)[J].植物学报（英文版）,2022(08):1631-1645
A类：
CWMV,Furovirus,CP19K,dehydrogenases,GAPCs,tophagic,tophagy
B类：
Coat,protein,Chinese,wheat,mosaic,upregulates,interacts,cytosolic,glyceraldehyde,phosphate,negative,plant,autophagy,promote,infection,Autophagy,intracellular,degradation,mechanism,involved,antiviral,defense,but,strategies,employed,by,viruses,counteract,related,remain,unknown,majority,Herein,we,describe,how,genus,interferes,enhances,its,Nicotiana,benthamiana,Yeast,two,hybrid,screening,vivo,vitro,assays,revealed,that,kDa,coat,regulators,which,ATG3,key,also,directly,possibly,leading,formation,complex,interaction,appeared,intensify,binding,Moreover,transcripts,reduced,activities,Accordingly,silencing,genes,transgenic,accumulation,whereas,overexpression,enhanced,Overall,our,results,suggest,through,promotion,utilization,role
AB值：
0.440974
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
