典型文献
Kiwifruit (Actinidia chinensis 'Hongyang') cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana
文献摘要：
Ascorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic acid (AsA) accumulation. This study aims to identify and characterize two cytosolic APX genes (AcAPX1 and AcAPX2) derived from A. chinensis 'Hongyang'. The constitutive expression pattern was determined for both AcAPX1 and AcAPX2, and showed relatively higher expression abundances of AcAPX1 in leaf and AcAPX2 in root. Transcript levels of AcAPX1 and AcAPX2 were increased in kiwifruit roots treated with NaCl. Subcellular localization assays using GFP-fusion proteins in Arabidopsis protoplasts showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Recombinant AcAPX1 or AcAPX2 proteins were successfully expressed in the prokaryotic expression system and their individual ascorbate peroxidase activities were determined. Finally, constitutive over-expression of AcAPX1 or AcAPX2 could dramatically increase total AsA, glutathione level and salinity tolerance under NaCl stress in Arabidopsis thaliana. Our findings revealed that cytosolic AcAPX1/2 may play an important protective role in the responses to unfavorable environmental stimuli in kiwifruit.
文献关键词：
中图分类号：
作者姓名：
GUO Xiu-hong;HE Yan;ZHANG Yu;WANG Yi;HUANG Sheng-xiong;LIU Yong-sheng;LI Wei
作者机构：
Key Laboratory for Bio-resource and Eco-environment of Ministry of Education,College of Life Sciences/State Key Laboratory of Hydraulics and Mountain River Engineering,Sichuan University,Chengdu 610064,P.R.China;School of Horticulture,Anhui Agricultural University,Hefei 230036,P.R.China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,P.R.China
文献出处：
引用格式：
[1]GUO Xiu-hong;HE Yan;ZHANG Yu;WANG Yi;HUANG Sheng-xiong;LIU Yong-sheng;LI Wei-.Kiwifruit (Actinidia chinensis 'Hongyang') cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana)[J].农业科学学报（英文）,2022(04):1058-1070
A类：
Kiwifruit,Hongyang,AcAPX1,AcAPX2,Ascorbate,APXs
B类：
Actinidia,chinensis,cytosolic,ascorbate,peroxidases,enhance,salinity,tolerance,Arabidopsis,thaliana,plays,key,role,scavenging,reactive,oxygen,species,ROS,higher,plants,However,there,very,little,information,available,kiwifruit,which,economically,nutritionally,important,horticultural,crop,exceptionally,ascorbic,acid,AsA,accumulation,This,study,aims,identify,characterize,two,genes,derived,from,constitutive,expression,pattern,was,determined,both,showed,relatively,abundances,leaf,Transcript,levels,were,increased,roots,treated,NaCl,Subcellular,localization,assays,using,GFP,fusion,proteins,protoplasts,that,are,targeted,Recombinant,successfully,expressed,prokaryotic,system,their,individual,activities,Finally,over,could,dramatically,total,glutathione,under,stress,Our,findings,revealed,may,protective,responses,unfavorable,environmental,stimuli
AB值：
0.486518
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
