典型文献
Overexpression of the ThTPS gene enhanced salt and osmotic stress tolerance in Tamarix hispida
文献摘要:
Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abi-otic stresses.Trehalose-6-phosphate synthase (TPS) plays important roles in trehalose metabolism and signaling.In this study,the full-length cDNA of ThTPS was cloned from Tamarix hispida Willd.A phylogenetic tree includ-ing ThTPS and 11 AtTPS genes from Arabidopsis indicated that the ThTPS protein had a close evolutionary relationship with AtTPS7.However,the function of AtTPS7 has not been determined.To analyze the abiotic stress tolerance function of ThTPS,the expression of ThTPS in T.hispida under salt and drought stress and JA,ABA and GA3 hormone stimu-lation was monitored by qRT-PCR.The results show that ThTPS expression was clearly induced by all five of these treatments at one or more times,and salt stress caused par-ticularly strong induction of ThTPS in the roots of T.hispida.The ThTPS gene was transiently overexpressed in T.his-pida.Both physiological indexes and staining results showed that ThTPS gene overexpression increased salt and osmotic stress tolerance in T.hispida.Overall,the ThTPS gene can respond to abiotic stresses such as salt and drought,and its overexpression can significantly improve salt and osmotic tolerance.These findings establish a foundation to better understand the responses of TPS genes to abiotic stress in plants.
文献关键词:
中图分类号:
作者姓名:
Peilong Wang;Xiaojin Lei;Jiaxin Lü;Caiqiu Gao
作者机构:
State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University),Harbin 150040,People's Republic of China
文献出处:
引用格式:
[1]Peilong Wang;Xiaojin Lei;Jiaxin Lü;Caiqiu Gao-.Overexpression of the ThTPS gene enhanced salt and osmotic stress tolerance in Tamarix hispida)[J].林业研究(英文版),2022(01):299-308
A类:
ThTPS,disaccharide,abi,AtTPS,AtTPS7,pida
B类:
Overexpression,enhanced,salt,osmotic,tolerance,Tamarix,hispida,Trehalose,reducing,high,stability,strong,water,absorption,properties,that,improve,resistance,organisms,various,stresses,phosphate,synthase,plays,important,roles,trehalose,metabolism,signaling,In,this,study,full,length,cDNA,was,cloned,from,Willd,phylogenetic,tree,includ,genes,Arabidopsis,indicated,protein,had,close,evolutionary,relationship,However,function,not,been,determined,To,analyze,abiotic,drought,JA,ABA,GA3,hormone,stimu,monitored,by,qRT,results,clearly,induced,five,these,treatments,more,times,caused,par,ticularly,induction,roots,transiently,overexpressed,Both,physiological,indexes,staining,showed,overexpression,increased,Overall,respond,such,its,significantly,These,findings,establish,foundation,better,understand,responses,plants
AB值:
0.462752
相似文献
机标中图分类号,由域田数据科技根据网络公开资料自动分析生成,仅供学习研究参考。