Phosphocholine (PCho) is an intermediate me-tabolite of nonplastid plant membranes that is essential for salt tolerance. However, how PCho metabolism modulates response to salt stress remains unknown. Here, we characterize the role of phosphoethanolamine N-methyltransferase 1 (PMT1) in salt stress tolerance in Arabidopsis thaliana using a T-DNA insertional mutant, gene-editing alleles, and complemented lines. The pmt1 mutants showed a severe inhibition of root elon-gation when exposed to salt stress, but exoge-nous ChoCl or lecithin rescued this defect. pmt1 also displayed altered glycerolipid metabolism under salt stress, suggesting that glycerolipids contribute to salt tolerance. Moreover, pmt1 mu-tants exhibited altered reactive oxygen species (ROS) accumulation and distribution, reduced cell division activity, and disturbed auxin distribution in the primary root compared with wild-type seedlings. We show that PMT1 expression is in-duced by salt stress and relies on the abscisic acid (ABA) signaling pathway, as this induction was abolished in the aba2-1 and pyl112458 mu-tants. However, ABA aggravated the salt sensi-tivity of the pmt1 mutants by perturbing ROS distribution in the root tip. Taken together, we propose that PMT1 is an important phosphoe-thanolamine N-methyltransferase participating in root development of primary root elongation under salt stress conditions by balancing ROS pro-duction and distribution through ABA signaling.

Qi Yu He;Jian Feng Jin;He Qiang Lou;Feng Feng Dang;Ji Ming Xu;Shao Jian Zheng;Jian Li Yang

State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310058,China;State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University,Guangzhou 510642,China

植物学报（英文版）

[1]Qi Yu He;Jian Feng Jin;He Qiang Lou;Feng Feng Dang;Ji Ming Xu;Shao Jian Zheng;Jian Li Yang-.Abscisic acid-dependent PMT1 expression regulates salt tolerance by alleviating abscisic acid-mediated reactive oxygen species production in Arabidopsis)[J].植物学报（英文版）,2022(09):1803-1820

PMT1,Phosphocholine,PCho,tabolite,nonplastid,insertional,pmt1,elon,exoge,ChoCl,aba2,pyl112458,phosphoe,thanolamine

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