典型文献
The transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin levels in Artemisia annua
文献摘要：
The important antimalarial drug artemisinin is bio-synthesized and stored in Artemisia annua glandular trichomes and the artemisinin content correlates with trichome density; however, the factors affecting trichome development are largely unknown. Here, we demonstrate that the A. annua R2R3 MYB transcription factor TrichomeLess Regulator 1 (TLR1) negatively regulates trichome development. In A. annua, TLR1 overexpression lines had 44.7％–64.0％ lower trichome density and 11.5％–49.4％lower artemisinin contents and TLR1-RNAi lines had 33％–93.3％ higher trichome density and 32.2％– 84.0％ higher artemisinin contents compared with non-transgenic controls. TLR1 also negatively regu-lates the expression of anthocyanin biosynthetic pathway genes in A. annua. When heterologously expressed in Arabidopsis thaliana, TLR1 interacts with GLABROUS3a, positive regulator of trichome development, and represses trichome development. Yeast two-hybrid and pull-down assays indicated that TLR1 interacts with the WUSCHEL homeobox (WOX) protein AaWOX1, which interacts with the LEAFY-like transcription factor TLR2. TLR2 over-expression in Arabidopsis and A. annua showed that TLR2 reduces trichome development by reducing gibberellin levels. Furthermore, artemisinin contents were 19％–43％ lower in TLR2-overexpressing A. annua plants compared to controls. These data indicate that TLR1 and TLR2 negatively regulate tri-chome density by lowering gibberellin levels and may enable approaches to enhance artemisinin yields.
文献关键词：
中图分类号：
作者姓名：
Zongyou Lv;JinXing Li;Shi Qiu;Fei Qi;Hang Su;Qitao Bu;Rui Jiang;Kexuan Tang;Lei Zhang;Wansheng Chen
作者机构：
Research and Development Center of Chinese Medicine Resources and Biotechnology,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;Department of Pharmaceutical Botany,School of Pharmacy,Second Military Medical University,Shanghai 200433,China;Joint International Research Laboratory of Metabolic&Developmental Sciences,Key Laboratory of Urban Agriculture(South)Ministry of Agriculture,Plant Biotechnology Research Center,Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,Shanghai Jiao Tong University,Shanghai 200240,China;State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China
文献出处：
引用格式：
[1]Zongyou Lv;JinXing Li;Shi Qiu;Fei Qi;Hang Su;Qitao Bu;Rui Jiang;Kexuan Tang;Lei Zhang;Wansheng Chen-.The transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin levels in Artemisia annua)[J].植物学报（英文版）,2022(06):1212-1228
A类：
TrichomeLess,GLABROUS3a,AaWOX1,chome
B类：
transcription,factors,TLR1,TLR2,negatively,density,artemisinin,levels,Artemisia,annua,important,antimalarial,drug,synthesized,stored,glandular,trichomes,correlates,however,affecting,development,largely,unknown,Here,demonstrate,that,R2R3,MYB,Regulator,regulates,In,overexpression,lines,had,contents,RNAi,higher,compared,transgenic,controls,also,anthocyanin,biosynthetic,pathway,genes,When,heterologously,expressed,Arabidopsis,thaliana,interacts,positive,regulator,represses,Yeast,two,hybrid,pull,down,assays,indicated,WUSCHEL,homeobox,protein,which,LEAFY,like,showed,reduces,by,reducing,gibberellin,Furthermore,were,overexpressing,plants,These,data,lowering,may,enable,approaches,enhance,yields
AB值：
0.419335
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
