Background: The traditional Chinese medicine NiuBeiXiaoHe (NBXH) extract and Chinese medicine preparation JieHeWan (JHW) exhibit anti-tuberculosis (TB) effects. The anti-TB effect of NBXH was compared with that of JHW to elucidate the mechanism of action of NBXH.Methods: BALB/c mice aged 6-8 weeks were randomly divided into a normal control group, TB model group, JHW treatment group, and NBXH treatment group. After 3 and 13 weeks of treatment, the therapeutic effect in each group was evaluated by comparing lung histopathology, lung and liver colony counts, the number of spots representing effector T cells secreting IFN-γ in an enzyme-linked immunospot assay (ELISPOT), and the levels of Th1, Th2, and Th17 cytokines, which were measured by a cytometric bead array (CBA). Mouse RNA samples were subjected to transcriptome sequencing. Results: After 13 weeks of treatment, the mean histopathological lesion area of the NBXH group was significantly smaller than that of the TB model group (P<0.05). Compared with those in the TB model group, the lung colony counts in the JHW and NBXH groups were significantly decreased (P<0.05), and the IL-2 and IL-4 levels in the NBXH group were significantly increased (P<0.05). NBXH partly restored significant changes in gene expression caused by Mycobacterium tuberculosis (M. tuberculosis) infection. According to GO and KEGG analyses, the changes in biological process (BP), cell composition (CC) and molecular function (MF) terms and in signaling pathways caused by NBXH and JHW treatment were not completely consistent, but they were mainly related to the immune response and inflammatory response in the mouse TB model. Conclusions: NBXH had therapeutic effects similar to those of JHW in improving lung histopathology, reducing lung colony counts, and regulating the levels of cytokines. NBXH restored significant changes in gene expression and repaired cell damage caused by M. tuberculosis infection by regulating immune-related pathways, which clarified the mechanism of action of NBXH.

Li-Yao Duan;Yan Liang;Wen-Ping Gong;Yong Xue;Jie Mi;Jie Wang;Lan Wang;Zai-Xing Jia;Hong Lei;Yu-Mei Liang;Jun Liu;Yue Zheng;Xue-Qiong Wu

Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Tuberculosis Research Institute, the 8th Medical Center,Chinese PLA General Hospital, Beijing 100091, China;Hebei North University, Zhangjiakou 075000, Hebei, China;Clinical Laboratory,the 8th Medical Center, Chinese PLA General Hospital, Beijing 100091,China;Pathology Department, the 8th Medical Center, Chinese PLA General Hospital, Beijing 100091, China;Guangdong Qifang Pharmaceutical Co., Ltd, Guangzhou 510075, China

军事医学研究（英文）

[1]Li-Yao Duan;Yan Liang;Wen-Ping Gong;Yong Xue;Jie Mi;Jie Wang;Lan Wang;Zai-Xing Jia;Hong Lei;Yu-Mei Liang;Jun Liu;Yue Zheng;Xue-Qiong Wu-.Comparative study on the anti-tuberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan)[J].军事医学研究（英文）,2022(02):164-183

NiuBeiXiaoHe,JieHeWan,NBXH,JHW

Comparative,study,anti,tuberculous,mechanism,traditional,Chinese,medicines,extract,Background,preparation,exhibit,tuberculosis,TB,effects,was,compared,that,elucidate,action,Methods,BALB,mice,aged,weeks,were,randomly,divided,into,normal,control,model,treatment,After,therapeutic,each,evaluated,by,comparing,lung,histopathology,liver,colony,counts,number,spots,representing,effector,cells,secreting,IFN,enzyme,linked,immunospot,assay,ELISPOT,levels,Th2,Th17,cytokines,which,measured,cytometric,bead,array,CBA,Mouse,samples,subjected,transcriptome,sequencing,Results,mean,histopathological,lesion,area,significantly,smaller,than,Compared,those,groups,decreased,increased,partly,restored,changes,gene,expression,caused,Mycobacterium,infection,According,analyses,biological,process,composition,CC,molecular,function,MF,terms,signaling,pathways,not,completely,consistent,but,they,mainly,related,immune,response,inflammatory,mouse,Conclusions,had,similar,improving,reducing,regulating,repaired,damage,clarified

0.435199