Sentinel lymph node(SLN)mapping and tumor-boundary delineation play a key role in cancer surgery,as they have great potential to reduce surgical interven-tion and increase relapse-free survival rates of patients.The autofluorescence imaging(AFI)method can improve the efficiency of tumor delineation and optimize the scope of surgical intervention,but there are still no fluorescent drugs that can be used with such a method to form a hybrid imaging technique.Another problem is bleaching when fluorescent dyes are conjugated with folic acid.This study reports,for the first time,nanosensors with excellent photo-stability and compatibility with endoscopes for AFI,which makes simultaneous hybrid imaging possible.After functionalization of the quantum dot(QD)surfaces,we found that they bound effectively to MCF-7 cancer cells.The diagnostic value of simultan-eous hybrid imaging using common AFI equipment in delineating tumor boundaries and mapping SLN can reduce the cost of diagnosis and increase its reliability.

Denis KUZNETSOV;Sergey DEZHUROV;Dmitri KRYLSKY;Valery NOVIKOV;Valery NESCHISLIAEV;Anastasiia KUZNETSOVA

G.N.Gabrichevsky Scientific and Research Institute of Epidemiology and Microbiology,Moscow 125212,Russia;Perm State Pharmaceutical Academy,Perm 614990,Russia;Research Institute of Applied Acoustics,Center of High Technologies,Dubna 141980,Russia;Perm State Medical University,Perm 614000,Russia;Perm State Humanitarian Pedagogical University,Perm 614990,Russia

浙江大学学报（英文版）（B辑：生物医学和生物技术）

[1]Denis KUZNETSOV;Sergey DEZHUROV;Dmitri KRYLSKY;Valery NOVIKOV;Valery NESCHISLIAEV;Anastasiia KUZNETSOVA-.Use of folic acid nanosensors with excellent photostability for hybrid imaging)[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2022(09):784-790

Use,folic,acid,nanosensors,excellent,photostability,hybrid,imaging,Sentinel,lymph,node,SLN,mapping,tumor,boundary,delineation,play,key,role,cancer,surgery,they,have,great,potential,reduce,surgical,increase,relapse,free,survival,rates,patients,autofluorescence,AFI,method,improve,efficiency,optimize,intervention,but,there,are,still,fluorescent,drugs,that,be,used,such,form,technique,Another,problem,bleaching,when,dyes,conjugated,This,study,reports,first,compatibility,endoscopes,which,makes,simultaneous,possible,After,functionalization,quantum,dot,QD,surfaces,we,found,effectively,MCF,cells,diagnostic,value,using,common,equipment,delineating,boundaries,cost,diagnosis,its,reliability

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