Chlorella meal is a potential protein source for aquafeeds.However,the physiological response of carnivorous fish fed Chlorella meal remains elusive.This study evaluated the effects of replacing dietary fish meal with Chlorella meal on growth performance,pigmentation,and liver health in largemouth bass.Five diets were formulated to replace dietary fish meal of 0％(C0,control),25％(C25),50％(C50),75％(C75),and 100％(C100)with Chlorella meal,respectively.Total 300 fish(17.6±0.03 g)were randomly assigned to 15 tanks(3 tanks/group).Fish were fed the experimental diet twice daily for 8 weeks.The increased dietary Chlorella meal quadratically influenced the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and feed intake(FI),which were significantly lower in the C100 group than in the other groups(P＜0.05).The feed conversion ratio(FCR)increased linearly or quadratically with dietary Chlorella meal.Dietary Chlorella meal linearly or quadratically increased the lutein content of plasma,liver,and dorsal muscle of largemouth bass(P＜0.05).Compared to the C0 group,all sup-plemented Chlorella meal groups significantly improved the yellowness(b*)of the dorsal body(1.5 to 2.0 fold),abdominal body(1.5 to 1.8 fold),and dorsal muscle(3.8 to 5.4 fold)of largemouth bass(P＜0.05).In addition,compared to the C0 group,the liver vacuolation area of fish was significantly increased in the C75 and C100 groups(P＜0.05).Transcriptional levels of apoptosis-related genes of β-cell lymphoma-2(bcl2),caspase-9-like(casp9),and caspase-3a(casp3)were markedly upregulated(0.9 to 1.6 fold)in the C100 group compared to the C0 group(P＜0.05).Based on the quadratic regression analysis between FBW,WGR,or SGR and dietary Chlorella meal level,largemouth bass had the best growth when replacing 31.7％to 32.6％of fish meal with 15.03％to 15.43％dietary Chlorella meal.The present results indicated that dietary supplementation with Chlorella meal(11.85％to 47.45％)significantly enhanced the pigmentation;however,total replacement of fish meal(40％)with Chlorella meal(47.45％)caused growth retardation,apoptosis,and liver damage in largemouth bass.

Longwei Xi;Qisheng Lu;Yulong Liu;Jingzhi Su;Wen Chen;Yulong Gong;Dong Han;Yunxia Yang;Zhimin Zhang;Junyan Jin;Haokun Liu;Xiaoming Zhu;Shouqi Xie

State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed,Wuhan 430072,China;The Innovative Academy of Seed Design,Chinese Academy of Sciences,Wuhan 430072,China

动物营养（英文）

[1]Longwei Xi;Qisheng Lu;Yulong Liu;Jingzhi Su;Wen Chen;Yulong Gong;Dong Han;Yunxia Yang;Zhimin Zhang;Junyan Jin;Haokun Liu;Xiaoming Zhu;Shouqi Xie-.Effects of fish meal replacement with Chlorella meal on growth performance,pigmentation,and liver health of largemouth bass(Micropterus salmoides))[J].动物营养（英文）,2022(03):26-40

largemouth,aquafeeds,C75,yellowness,vacuolation,casp9,casp3

Effects,fish,meal,replacement,Chlorella,growth,performance,pigmentation,liver,health,bass,Micropterus,salmoides,potential,protein,source,However,physiological,response,carnivorous,fed,remains,elusive,This,study,evaluated,effects,replacing,dietary,Five,diets,were,formulated,C0,control,C25,C50,C100,respectively,Total,randomly,assigned,tanks,Fish,experimental,twice,daily,weeks,increased,quadratically,influenced,final,body,weight,FBW,gain,rate,WGR,specific,SGR,intake,FI,which,significantly,lower,than,other,groups,conversion,ratio,FCR,linearly,Dietary,lutein,content,plasma,dorsal,muscle,Compared,plemented,improved,fold,abdominal,In,addition,compared,area,was,Transcriptional,levels,apoptosis,related,genes,cell,lymphoma,bcl2,caspase,like,3a,markedly,upregulated,Based,regression,analysis,between,had,best,when,present,results,indicated,that,supplementation,enhanced,however,total,caused,retardation,damage

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