Iso-nitrogenous and iso-lipidic diets containing 0％,3％,6％,9％,and 12％hydrolyzed porcine mucosa(namely,HPM0,HPM3,HPM6,HPM9,and HPM12)were prepared to evaluate their effects on the growth performance,muscle nutrition composition,texture property,and gene expression related to muscle growth of hybrid groupers(Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂).Groupers were fed to apparent satiation at 08:00 and 16:00 every day for a total of 56 days.It was found that the weight gain percentage in the HPM0,HPM3,and HPM6 groups did not differ(P＞0.05).The cooking loss and drip loss of the dorsal muscle in the HPM3 group were lower than those in the HPM6 and HPM9 groups(P＜0.05).The hardness and chewiness of the dorsal muscle in the HPM3 group were higher than those in the HPM0,HPM9,and HPM12 groups(P＜0.05).The gumminess in the HPM3 group was higher than that in the HPM9 and HPM12 groups(P＜0.05).The total essential amino acid content of the dorsal muscle in the HPM12 group was higher than that in the HPM0 group(P＜0.05).The contents of total n-3 polyunsaturated fatty acid and total n-3 highly unsaturated fatty acid,as well as the ratio of n-3/n-6 polyunsaturated fatty acid in the dorsal muscle was higher in the HPM0 group than in all other groups(P＜0.05).The relative expressions of gene myogenic factor 5,myocyte enhancer factor 2c,myocyte enhancer factor 2a,myosin heavy chain,transforming growth factor-beta 1(TGF-β1),and follistatin(FST)were the highest in the dorsal muscle of the HPM3 group.The results indicated that the growth per-formance of hybrid grouper fed a diet with 6％HPM and 27％fish meal was as good as that of the HPM0 group.When fish ingested a diet containing 3％HPM,the expression of genes TGF-β1 and FST involved in muscle growth were upregulated,and then the muscle quality related to hardness and chewiness were improved.An appropriate amount of HPM could be better used in grouper feed.

Xuanyi Yang;Xinyan Zhi;Ziling Song;Guanghui Wang;Xumin Zhao;Shuyan Chi;Beiping Tan

Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang),Zhanjiang 524025,China;Yichang Huatai Biological Technology Co.,Ltd.,Yichang 443500,China;Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province,Zhanjiang 524088,China

动物营养（英文）

[1]Xuanyi Yang;Xinyan Zhi;Ziling Song;Guanghui Wang;Xumin Zhao;Shuyan Chi;Beiping Tan-.Flesh quality of hybrid grouper(Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂)fed with hydrolyzed porcine mucosa-supplemented low fishmeal diet)[J].动物营养（英文）,2022(01):114-124

Flesh,lipidic,HPM0,HPM3,HPM6,HPM9,HPM12,groupers,Groupers,satiation,gumminess

quality,hybrid,Epinephelus,fuscoguttatus,lanceolatus,fed,hydrolyzed,porcine,mucosa,supplemented,fishmeal,Iso,nitrogenous,iso,diets,containing,namely,were,prepared,evaluate,their,effects,growth,performance,muscle,nutrition,composition,texture,property,related,apparent,every,total,days,It,was,found,that,weight,gain,percentage,groups,did,not,differ,cooking,loss,drip,dorsal,lower,than,those,hardness,chewiness,higher,essential,amino,acid,contents,polyunsaturated,fatty,highly,well,ratio,all,other,relative,expressions,myogenic,myocyte,enhancer,2c,2a,myosin,heavy,chain,transforming,beta,TGF,follistatin,FST,highest,results,indicated,good,When,ingested,genes,involved,upregulated,then,improved,An,appropriate,amount,could,better,used,feed

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