OBJECTIVE:To explore the effect of electroacupuncture(EA)on rats with chronic fallopian tube inflammation and its potential mechanisms.METHODS:Thirty-six female Sprague-Dawley rats were divided into Control,Model and EA groups.The pathological morphology of the fallopian tubes was observed by hematoxylin-eosin(HE)and Masson staining.The results of transforming growth factor-β1(TGF-β1),P38 mitogen-activated protein kinase(MAPK),phosphorylation(p)-p38MAPK in rat oviduct tissues were detected by immunohistochemistry.Results of P38MAPK,p-P38MAPK and TGF-β1 in rat oviduct tissues were detected by immunofluorescence.The expression level of p38MAPK,p-P38MAPK,TGF-β1 protein in rats was detected by Western blot.Quantitative real-time polymerase chain reaction(RT-qPCR)was used to detect mRNA expression levels of TGF-β1.RESULTS:It found that collagen fibers counts decreased significantly in EA group compared to Model group.The phosphorylation of P38MAPK in EA group was significantly reduced compared to Model group.The serum TGF-β1 expressions in EA group increased decreased significantly.CONCLUSION:Electroacupuncture was able to attenuate chronic salpingitis through down-regulating TGF-β1/MAPK signaling pathway.

HU Xijiao;CHENG Yinglong;KANG Huanan;LI Shuoxi;WANG Yawen;LIU Jinzhe;SUN Yiming;LIU Li

Department of obstetrics and gynecology,the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Heilongjiang 150001,China;Deapartment of Hysteroscopy chamber,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Midwifery Teaching and Research Section,Heilongjiang University of Chinese Medicine Jiamusi College,Harbin 150040,China;The department of obstetrics and gynecology,the First Affiliated Hospital of Henan University of Chinese Medicine,Henan 450000,China;Department of Chinese Pharmacy,School of Pharmacy,Guizhou University of Chinese Medicine,Guizhou 550000,China;Andrology department Heilongjiang Academy of Traditional Chinese Medicine,Harbin 150036,China;Endoscopy room,the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Hysteroscopy Department,Harbin 150040,China

中医杂志（英文版）

[1]HU Xijiao;CHENG Yinglong;KANG Huanan;LI Shuoxi;WANG Yawen;LIU Jinzhe;SUN Yiming;LIU Li-.Electroacupuncture attenuates chronic salpingitis via transforming growth factor-β1/p38 mitogen-activated protein kinase signaling pathway)[J].中医杂志（英文版）,2022(05):781-787

salpingitis,oviduct

Electroacupuncture,attenuates,chronic,via,transforming,growth,mitogen,activated,protein,kinase,signaling,pathway,OBJECTIVE,To,explore,effect,electroacupuncture,EA,rats,fallopian,inflammation,its,potential,mechanisms,METHODS,Thirty,six,female,Sprague,Dawley,were,divided,into,Control,Model,groups,pathological,morphology,tubes,was,observed,by,hematoxylin,eosin,HE,Masson,staining,results,TGF,phosphorylation,p38MAPK,tissues,detected,immunohistochemistry,Results,P38MAPK,immunofluorescence,blot,Quantitative,real,polymerase,chain,reaction,qPCR,used,levels,RESULTS,It,found,that,collagen,fibers,counts,decreased,significantly,compared,reduced,serum,expressions,increased,CONCLUSION,able,through,down,regulating

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