Steroid saponins are secondary metabolites with multiple medicinal values that are found in large quantities in natural medicines,especially Vernonia amygdalina,a famous nature medicine for the treatment of tonsillitis,diabetes,pneumonia.The cur-rent study was designed to combine molecular networking(MN)with diagnostic ions for rapid identification of Δ7,9(11)stigmastane-type saponins which were the α-glucosidase inhibitory active substances in V.amygdalina.First,the α-glucosidase inhibitory activities of five Δ7,9(11)stigmastane-type steroid saponins that were previously isolated were screened,which indicated that the Δ7,9(11)stigmastane-type steroid saponin was one of the active constituents responsible for ameliorating diabetes.Furthermore,a strategy was proposed to identify stigmastane-type steroid saponins and verify the plausibility of derived fragmentation pathways by applying MN,MolNetEn-hancer and unsupervised substructure annotation(MS2LDA).Based on this strategy,other seven Δ7,9(11)stigmastane-type steroid saponins were identified from this plant.Our research provide scientific evidence for the antidiabetic potential of the steroid saponin-rich extract of V.amygdalina leaf.

GAO Juanjuan;ZHAO Mengling;SHAN Shujun;LI Yongyi;LUO Jun;LI Yi

School of Food Science and Pharmaceutical Engineering,Testing&Analysis Center,Nanjing Normal University,Nanjing 210023,China;Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines,China Pharma-ceutical University,Nanjing 210009,China

中国天然药物

[1]GAO Juanjuan;ZHAO Mengling;SHAN Shujun;LI Yongyi;LUO Jun;LI Yi-.Rapid identification of stigmastane-type steroid saponins from Ver-nonia amygdalina leaf based on a-glucosidase inhibiting activity and molecular networking)[J].中国天然药物,2022(11):846-853

stigmastane,nonia,amygdalina,Vernonia,tonsillitis,MolNetEn,hancer,MS2LDA

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