Baicalin is a natural active ingredient isolated from Scutellariae Radix that can cross the blood-brain barrier and exhibits neuroprotective effects on multiple central nervous system diseases. However, the mechanism behind the neuroprotective effects remains unclear. In this study, rat models of spinal cord injury were established using a modified Allen's impact method and then treated with intraperitoneal injection of Baicalin. The results revealed that Baicalin greatly increased the Basso, Beattie, Bresnahan Locomotor Rating Scale score, reduced blood-spinal cord barrier permeability, decreased the expression of Bax, Caspase-3, and nuclear factor κB, increased the expression of Bcl-2, and reduced neuronal apoptosis and pathological spinal cord injury. SH-SY5Y cell models of excitotoxicity were established by application of 10 mM glutamate for 12 hours and then treated with 40 μM Baicalin for 48 hours to investigate the mechanism of action of Baicalin. The results showed that Baicalin reversed tight junction protein expression tendencies (occludin and ZO-1) and apoptosis-related protein expression (Bax, Bcl-2, Caspase-3, and nuclear factor-κB), and also led to up-regulation of PI3K and Akt phosphorylation. These effects on Bax, Bcl-2, and Caspase-3 were blocked by pretreatment with the PI3K inhibitor LY294002. These findings suggest that Baicalin can inhibit blood-spinal cord barrier permeability after spinal cord injury and reduce neuronal apoptosis, possibly by activating the PI3K/Akt signaling pathway. This study was approved by Animal Ethics Committee of Xi'an Jiaotong University on March 6, 2014.

Rui Zhao

College of Pharmacy,Shaanxi University of Chinese Medicine,Xi'an,Shaanxi Province,China;Key Laboratory of the Ministry of Education for MedicinalResources and Natural Pharmaceutical Chemistry,National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China,College of Life Sciences,Shaanxi Normal University,Xi'an,Shaanxi Province,China;Translational Medicine Center,Hong Hui Hospital,Xi'an Jiaotong University,Xi'an,Shaanxi Province,China;Department of Pharmacy,Hong Hui Hospital,Xi'an Jiaotong University,Xi'an,Shaanxi Province,China

中国神经再生研究（英文版）

[1]Rui Zhao-.Baicalin attenuates blood-spinal cord barrier disruption and apoptosis through PI3K/Akt signaling pathway after spinal cord injury)[J].中国神经再生研究（英文版）,2022(05):1080-1087

Scutellariae

Baicalin,attenuates,blood,spinal,cord,barrier,disruption,apoptosis,through,PI3K,Akt,signaling,pathway,after,injury,natural,active,ingredient,isolated,from,Radix,that,can,cross,brain,exhibits,neuroprotective,effects,multiple,central,nervous,system,diseases,However,mechanism,behind,remains,unclear,In,this,study,rat,models,were,established,using,modified,Allen,impact,method,then,treated,intraperitoneal,injection,results,revealed,greatly,increased,Basso,Beattie,Bresnahan,Locomotor,Rating,Scale,score,reduced,permeability,decreased,expression,Bax,Caspase,nuclear,Bcl,neuronal,pathological,SH,SY5Y,cell,excitotoxicity,by,application,mM,glutamate,hours,investigate,action,showed,reversed,tight,junction,protein,tendencies,occludin,ZO,related,also,regulation,phosphorylation,These,blocked,pretreatment,inhibitor,LY294002,findings,suggest,possibly,activating,This,was,approved,Animal,Ethics,Committee,Xi,Jiaotong,University,March

0.540313