Blood-brain barrier (BBB) disruption underlies the vasogenic edema and neuronal cell death induced by acute ischemic stroke.Reducing this disruption has therapeutic potential.Transcranial focused ultrasound stimulation has shown neuromodulatory and neuroprotective effects in various brain diseases including ischemic stroke.Ultrasound stimulation can reduce inflammation and promote angiogenesis and neural circuit remodeling.However,its effect on the BBB in the acute phase of ischemic stroke is unknown.In this study of mice subjected to middle cerebral artery occlusion for 90 minutes,low-intensity low-frequency (0.5 MHz) transcranial focused ultrasound stimulation was applied 2,4,and 8 hours after occlusion.Ultrasound stimulation reduced edema volume,improved neurobehavioral outcomes,improved BBB integrity (enhanced tight junction protein ZO-1 expression and reduced IgG leakage),and reduced secretion of the inflammatory factors tumor necrosis factor-α and activation of matrix metalloproteinase-9 in the ischemic brain.Our results show that low-intensity ultrasound stimulation attenuated BBB disruption and edema formation,which suggests it may have therapeutic use in ischemic brain disease as a protector of BBB integrity.

Li-Dong Deng;Lin Qi;Qian Suo;Sheng-Ju Wu;Muyassar Mamtilahun;Ru-Bing Shi;Ze Liu;Jun-Feng Sun;Yao-Hui Tang;Zhi-Jun Zhang;Guo-Yuan Yang;Ji-Xian Wang

Department of Rehabilitation Medicine,Ruijin Hospital,School of Medicine,and Med-X Research Institute and School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai,China;Department of Neurology,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China

中国神经再生研究（英文版）

[1]Li-Dong Deng;Lin Qi;Qian Suo;Sheng-Ju Wu;Muyassar Mamtilahun;Ru-Bing Shi;Ze Liu;Jun-Feng Sun;Yao-Hui Tang;Zhi-Jun Zhang;Guo-Yuan Yang;Ji-Xian Wang-.Transcranial focused ultrasound stimulation reduces vasogenic edema after middle cerebral artery occlusion in mice)[J].中国神经再生研究（英文版）,2022(09):2058-2063

vasogenic,neuromodulatory

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