典型文献
Development of a multiprimer metabarcoding approach to understanding trophic interactions in agroecosystems
文献摘要：
To understand trophic interactions and the precise ecological role of each predatory species,it is important to know which arthropod and plant resources are used by generalist predators in agroecosystems.Molecular approaches,such as the use of high-throughput sequencing(HTS),play a key role in identifying these resources.This study develops a multiprimer metabarcoding approach for screening the most common trophic interactions of two predatory arthropods with contrasting morphologies,Rhagonycha fulva(Coleoptera:Cantharidae)and Anthocoris nemoralis(Hemiptera:Anthocoridae)collected from a peach crop.To reduce the time and cost of this metabarcoding approach,we first evaluated the effect of using two different predator-pools of different size(10 and 23 in-dividuals of the same species).We also used our system to analyze the performance of one and two primer pairs in the same library.Our results show that the analysis of 23 in-dividuals together with the use of two primer pairs in the same library optimize the HTS analysis.Using these best-performing conditions,we then analyzed the entire bodies of field-collected predators as well as the washing solutions used to clean the insect bodies.We were able to identify both gut content(i.e.,diet)and external pollen load(i.e.,on the in-sects'bodies).This study also demonstrates the importance of washing predatory insects'bodies prior to HTS analysis when the target species have a considerable size(＞10 mm)and hairy structures.This metabarcoding approach has significant potential for the study of trophic links in agriculture,revealing expected and unexpected trophic relationships.
文献关键词：
中图分类号：
作者姓名：
Ivan Batuecas;Oscar Alomar;Cristina Casta?e;Josep Pi?ol;Stéphane Boyer;Lorena Gallardo-Montoya;Nuria Agustí
作者机构：
IRTA,Sustainable Plant Protection,Cabrils,Barcelona,Spain;Universitat Autònoma Barcelona,Cerdanyola del Vallès,Spain;CREAF,Cerdanyola del Vallès,Spain;Institut de Recherche sur la Biologie de l'Insecte(IRBI),Tours University,Tours,France
文献出处：
引用格式：
[1]Ivan Batuecas;Oscar Alomar;Cristina Casta?e;Josep Pi?ol;Stéphane Boyer;Lorena Gallardo-Montoya;Nuria Agustí-.Development of a multiprimer metabarcoding approach to understanding trophic interactions in agroecosystems)[J].中国昆虫科学（英文版）,2022(04):1195-1210
A类：
multiprimer,Rhagonycha,Cantharidae,Anthocoris,nemoralis,Anthocoridae,sects
B类：
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AB值：
0.494192
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