典型文献
Characterization and mode of action analysis of black soldier fly(Hermetia illucens)larva-derived hemocytes
文献摘要：
With the growing importance of the black soldier fly(Hermetia illucens)for both sustainable food production and waste management as well as for science,a great de-mand of understanding its immune system arises.Here,we present the first description of the circulating larval hemocytes with special emphasis on uptake of microorganisms and distinguishing hemocyte types.With histological,zymographic,and cytometric methods and with a set of hemocyte binding lectins and antibodies,the hemocytes of H.illucens are identified as plasmatocytes,crystal cells,and putative prohemocytes.Total hemo-cyte counts(THC)are determined,and methods for THC determination are compared.Approximately 1100 hemocytes per microliter hemolymph are present in naive animals,while hemocyte density decreases dramatically shortly after wounding,indicating a role of hemocytes in response to wounding(and immune response in general).The determina-tion of the relative abundance of each hemocyte type(differential hemocyte count,DHC)revealed that plasmatocytes are highly abundant,whereas prohemocytes and crystal cells make up only a small percentage of the circulating cells.Plasmatocytes are not only the most abundant but also the professional phagocytes in H.illucens.They rapidly engulf and take up bacteria both in vivo and in vitro,indicating a very potent cellular defense against invading pathogens.Larger bioparticles such as yeasts are also removed from cir-culation by phagocytosis,but slower than bacteria.This is the first analysis of the potent cellular immune response in the black soldier fly,and a first toolbox that helps to identify hemocyte(types)is presented.
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中图分类号：
作者姓名：
Yvette M.von Bredow;Ariane Müller;Philipp F.Popp;Denis Iliasov;Christoph-Rüdiger von Bredow
作者机构：
Justus-Liebig-Universit?t Gie?en,Institut für Allgemeine Zoologie und Entwicklungsbiologie,Zellul?re Erkennungs-und Ab-wehrprozesse,Gie?en,Germany;Technische Universit?t Dresden,Fakult?t Biologie,Institut für Zoologie,Dresden,Germany;Technische Universit?t Dresden,Fakult?t Biologie,Institut für Mikrobiologie,Dresden,Germany;Philipp F.Popp,Institute for Biology-Bacterial Physiology,Humboldt-Universit?t zu Berlin,Berlin,Germany
文献出处：
引用格式：
[1]Yvette M.von Bredow;Ariane Müller;Philipp F.Popp;Denis Iliasov;Christoph-Rüdiger von Bredow-.Characterization and mode of action analysis of black soldier fly(Hermetia illucens)larva-derived hemocytes)[J].中国昆虫科学（英文版）,2022(04):1071-1095
A类：
hemocytes,hemocyte,zymographic,plasmatocytes,prohemocytes,microliter,Plasmatocytes,engulf
B类：
Characterization,mode,action,analysis,black,soldier,fly,Hermetia,illucens,derived,With,growing,importance,both,sustainable,food,production,waste,management,well,science,great,mand,understanding,its,immune,system,arises,Here,first,description,circulating,larval,special,emphasis,uptake,microorganisms,distinguishing,types,histological,cytometric,methods,set,binding,lectins,antibodies,identified,crystal,cells,putative,Total,counts,THC,determined,determination,compared,Approximately,hemolymph,naive,animals,while,density,decreases,dramatically,shortly,after,wounding,indicating,role,response,general,relative,abundance,each,differential,DHC,revealed,that,highly,abundant,whereas,make,only,small,percentage,not,most,but,also,professional,phagocytes,They,rapidly,bacteria,vivo,vitro,very,potent,cellular,defense,against,invading,pathogens,Larger,bioparticles,such,yeasts,removed,from,culation,by,phagocytosis,slower,than,This,toolbox,helps,identify,presented
AB值：
0.501573
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