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典型文献
Nanocarrier-delivered dsRNA suppresses wing development of green peach aphids
文献摘要:
RNA interference(RNAi)has developed rapidly as a potential"green"pest management strategy.At present,most studies have focused on the screening of aphid lethal genes,whereas only a few studies have been conducted on wing development,which is crucial for aphid migration and plant-virus dissemination.Here,the Myzus persicae genes vestigial(vg)and Ultrabithorax(Ubx)related to wing development,were cloned.These two genes were expressed in various tissues of 3rd-instar winged aphids.The mRNA level of vg was high in 3rd-instar nymphs,whereas the expression level of Ubx was high in adults.The nanocarrier-mediated delivery system delivered double-stranded RNAs for aphid RNAi using topical and root applications.The expression levels of vg and Ubx were downregulated by 44.0%and 36.5%,respectively,using the topical application.The simultaneous RNAi of the two target genes caused 63.3%and 32.2%wing aberration rates using topical and root applications,respectively.The current study provided a promising method for controlling aphid migration to alleviate the spread of insect transmitted plant diseases.
文献关键词:
作者姓名:
Yun-Hui Zhang;Zhong-Zheng Ma;Hang Zhou;Zi-Jian Chao;Shuo Yan;Jie Shen
作者机构:
Department of Plant Biosecurity and MOA Key Lab of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University,Beijing,China
引用格式:
[1]Yun-Hui Zhang;Zhong-Zheng Ma;Hang Zhou;Zi-Jian Chao;Shuo Yan;Jie Shen-.Nanocarrier-delivered dsRNA suppresses wing development of green peach aphids)[J].中国昆虫科学(英文版),2022(03):669-682
A类:
Nanocarrier,vestigial,Ultrabithorax,Ubx
B类:
delivered,dsRNA,suppresses,development,green,peach,aphids,interference,RNAi,has,developed,rapidly,potential,pest,management,strategy,At,present,most,studies,have,focused,screening,lethal,genes,whereas,only,few,been,conducted,which,crucial,migration,plant,virus,dissemination,Here,Myzus,persicae,vg,related,were,cloned,These,two,expressed,various,tissues,3rd,instar,winged,was,high,nymphs,expression,adults,nanocarrier,mediated,delivery,system,double,stranded,RNAs,using,topical,root,applications,levels,downregulated,by,respectively,simultaneous,target,caused,aberration,rates,current,study,provided,promising,method,controlling,alleviate,spread,insect,transmitted,diseases
AB值:
0.535762
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