Background: Nucleobase–ascorbate transporters (NAT ), synonymously called nucleobase–cation symporter 2 (NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant different between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was con-ducted for the first time in cotton.Results: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups (groups I–IV ) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT mem-bers in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under different conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resist-ance to salt and drought stress through the virus-induced gene silencing ( VIGS) analysis.Conclusions: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analy-sis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to biotic and abiotic resistance for the improvement in cotton.

GUO Lixue;ZHAO Lanjie;LU Xuke;CHEN Xiugui;WANG Shuai;WANG Junjuan;WANG Delong;YIN Zujun;CHEN Chao;FAN Yapeng;ZHANG Yuexin;YE Wuwei

State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base,School of Agricultural Sciences,Zhengzhou University/Key Laboratory for Cotton Genetic Improvement,MOARA?,Anyang 455000,Henan,China

棉花研究（英文）

[1]GUO Lixue;ZHAO Lanjie;LU Xuke;CHEN Xiugui;WANG Shuai;WANG Junjuan;WANG Delong;YIN Zujun;CHEN Chao;FAN Yapeng;ZHANG Yuexin;YE Wuwei-.Characters and structures of the nucleobase–ascorbate transporters (NAT ) family genes in Gossypium hirsutum and their roles in responding to salt and drought stresses)[J].棉花研究（英文）,2022(02):1-15

nucleobase,Nucleobase,NCS2,NAT12,arboreum,GhNAT,GhNATs,GhNAT12

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