典型文献
The nonstructural protein 2C of Coxsackie B virus has RNA helicase and chaperoning activities
文献摘要：
RNA-remodeling proteins,including RNA helicases and chaperones,play vital roles in the remodeling of struc-tured RNAs.During viral replication,viruses require RNA-remodeling proteins to facilitate proper folding and/or re-folding the viral RNA elements.Coxsackieviruses B3(CVB3)and Coxsackieviruses B5(CVB5),belonging to the genus Enterovirus in the family Picornaviridae,have been reported to cause various infectious diseases such as hand-foot-and-mouth disease,aseptic meningitis,and viral myocarditis.However,little is known about whether CVB3 and CVB5 encode any RNA remodeling proteins.In this study,we showed that 2C proteins of CVB3 and CVB5 contained the conserved SF3 helicase A,B,and C motifs,and functioned not only as RNA helicase that unwound RNA helix bidirectionally in an NTP-dependent manner,but also as RNA chaperone that remodeled structured RNAs and facilitated RNA strand annealing independently of NTP.In addition,we determined that the NTPase activity and RNA helicase activity of 2C proteins of CVB3 and CVB5 were dependent on the presence of divalent metallic ions.Our findings demonstrate that 2C proteins of CVBs possess RNA-remodeling activity and underline the functional importance of 2C protein in the life cycle of CVBs.
文献关键词：
中图分类号：
作者姓名：
Ziyu Chen;Xiaobei Xiong;Yiyang Li;Muhan Huang;Yujie Ren;Di Wu;Yang Qiu;Mingzhou Chen;Ting Shu;Xi Zhou
作者机构：
State Key Laboratory of Virology,College of Life Sciences,Wuhan University,Wuhan,430072,China;State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China;University of Chinese Academy of Sciences,Beijing 100081,China
文献出处：
引用格式：
[1]Ziyu Chen;Xiaobei Xiong;Yiyang Li;Muhan Huang;Yujie Ren;Di Wu;Yang Qiu;Mingzhou Chen;Ting Shu;Xi Zhou-.The nonstructural protein 2C of Coxsackie B virus has RNA helicase and chaperoning activities)[J].中国病毒学,2022(05):656-663
A类：
chaperoning,Coxsackieviruses,unwound,CVBs
B类：
nonstructural,2C,has,activities,remodeling,proteins,including,helicases,chaperones,play,vital,roles,RNAs,During,viral,replication,require,proper,folding,elements,CVB3,CVB5,belonging,genus,Enterovirus,family,Picornaviridae,have,been,reported,cause,various,infectious,diseases,such,hand,foot,mouth,aseptic,meningitis,myocarditis,However,little,known,about,whether,encode,any,In,this,study,showed,that,contained,conserved,SF3,motifs,functioned,not,only,helix,bidirectionally,manner,but,also,remodeled,structured,facilitated,strand,annealing,independently,addition,determined,NTPase,activity,were,presence,divalent,metallic,ions,Our,findings,demonstrate,possess,underline,functional,importance,life,cycle
AB值：
0.501362
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