典型文献
A new luciferase immunoprecipitation system assay provided serological evidence for missed diagnosis of severe fever with thrombocytopenia syndrome
文献摘要：
Severe fever with thrombocytopenia syndrome(SFTS),caused by SFTS virus(SFTSV)infection,was first reported in 2010 in China with an initial fatality of up to 30％.The laboratory confirmation of SFTSV infection in terms of detection of viral RNA or antibody levels is critical for SFTS diagnosis and therapy.In this study,a new luciferase immunoprecipitation system(LIPS)assay based on pREN2 plasmid expressing SFTSV NP gene and tagged with Renilla luciferase(Rluc),was established and used to investigate the levels of antibody responses to SFTSV.Totally 464 serum samples from febrile patients were collected in the hospital of Shaoxing City in Zhejiang Province in 2019.The results showed that 82 of the 464 patients(17.7％)had antibody response to SFTSV,which were further supported by immunofluorescence assays(IFAs).Further,qRT-PCR and microneutralization tests showed that among the 82 positive cases,15 patients had viremia,10 patients had neutralizing antibody,and one had both(totally 26 patient).However,none of these patients were diagnosed as SFTS in the hospital probably because of their mild symptoms or subclinical manifestations.All the results indicated that at least the 26 patients having viremia or neutralizing antibody were the missed diagnosis of SFTS cases.The findings suggested the occurrence of SFTS and the SFTS incidence were higher than the reported level in Shaoxing in 2019,and that LIPS may provide an alternative strategy to confirm SFTSV infection in the laboratory.
文献关键词：
中图分类号：
作者姓名：
Shengyao Chen;Minjun Xu;Xiaoli Wu;Yuan Bai;Junming Shi;Min Zhou;Qiaoli Wu;Shuang Tang;Fei Deng;Bo Qin;Shu Shen
作者机构：
State Key Laboratory of Virology and National Virus Resource Center,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shaoxing People's Hospital,Zhejiang University School of Medicine,Shaoxing,312000,China;Shaoxing Women and Children's Hospital,No.305 East Street Road,Shaoxing,312000,China
文献出处：
引用格式：
[1]Shengyao Chen;Minjun Xu;Xiaoli Wu;Yuan Bai;Junming Shi;Min Zhou;Qiaoli Wu;Shuang Tang;Fei Deng;Bo Qin;Shu Shen-.A new luciferase immunoprecipitation system assay provided serological evidence for missed diagnosis of severe fever with thrombocytopenia syndrome)[J].中国病毒学,2022(01):107-114
A类：
pREN2,IFAs
B类：
new,luciferase,immunoprecipitation,system,provided,serological,evidence,missed,diagnosis,severe,fever,thrombocytopenia,syndrome,Severe,caused,by,virus,SFTSV,infection,was,first,reported,China,initial,fatality,laboratory,confirmation,terms,detection,viral,antibody,levels,critical,therapy,In,this,study,LIPS,plasmid,expressing,NP,gene,tagged,Renilla,Rluc,established,investigate,responses,Totally,serum,samples,from,febrile,patients,were,collected,hospital,Shaoxing,City,Zhejiang,Province,results,showed,that,had,which,further,supported,immunofluorescence,assays,Further,qRT,microneutralization,tests,among,positive,cases,viremia,neutralizing,both,totally,However,none,these,diagnosed,probably,because,their,mild,symptoms,subclinical,manifestations,All,indicated,least,having,findings,suggested,occurrence,incidence,higher,than,may,alternative,strategy
AB值：
0.503834
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