典型文献
An LTR retro transposon in the promoter of a PsMYB10.2 gene associated with the regulation of fruit flesh color in Japanese plum
文献摘要：
Japanese plums exhibit wide diversity of fruit coloration.The red to black hues are caused by the accumulation of anthocyanins,while their absence results in yellow,orange or green fruits.In Prunus,MYB10 genes are determinants for anthocyanin accumulation.In peach,QTLs for red plant organ traits map in an LG3 region with three MYB10 copies(PpMYB10.1,PpMYB10.2 and PpMYB10.3).In Japanese plum the gene copy number in this region differs with respect to peach:there are at least three copies of PsMYB10.1,with the expression of one of them(PsMYB10.1a)correlating with fruit skin color.The objective of this study was to determine a possible role of LG3-PsMYB10 genes in the natural variability of the flesh color trait and to develop a molecular marker for marker-assisted selection(MAS).We explored the variability within the LG3-PsMYB10 region using long-range sequences obtained in previous studies through CRISPR-Cas9 enrichment sequencing.We found that the PsMYB10.2 gene was only expressed in red flesh fruits.Its role in promoting anthocyanin biosynthesis was validated by transient overexpression in Japanese plum fruits.The analysis of long-range sequences identified an LTR retrotransposon in the promoter of the expressed PsMYB10.2 gene that explained the trait in 93.1％of the 145 individuals analyzed.We hypothesize that the LTR retrotransposon may promote the PsMYB10.2 expression and activate the anthocyanin biosynthesis pathway.We propose for the first time the PsMYB10.2 gene as candidate for the flesh color natural variation in Japanese plum and provide a molecular marker for MAS.
文献关键词：
中图分类号：
作者姓名：
Arnau Fiol;Sergio García;Christian Dujak;Igor Pacheco;Rodrigo Infante;Maria José Aranzana
作者机构：
Centre for Research in Agricultural Genomics(CRAG)CSIC-IRTA-UAB-UB,Campus UAB,Bellaterra,08193 Barcelona,Spain;Instituto de Nutrición y Tecnología de Alimentos(INTA),Universidad de Chile,El Líbano 5524,Santiago,Chile;Departamento de Producción Agrícola,Facultad de Ciencias Agronómicas,Universidad de Chile,Santa Rosa 11315,Santiago,Chile;IRTA(Institut de Recerca i Tecnologia Agroalimentàries),Barcelona,Spain
文献出处：
引用格式：
[1]Arnau Fiol;Sergio García;Christian Dujak;Igor Pacheco;Rodrigo Infante;Maria José Aranzana-.An LTR retro transposon in the promoter of a PsMYB10.2 gene associated with the regulation of fruit flesh color in Japanese plum)[J].园艺研究（英文）,2022(01):265-276
A类：
PsMYB10,plum,plums,PpMYB10,retrotransposon
B类：
An,LTR,promoter,associated,regulation,flesh,Japanese,exhibit,wide,diversity,coloration,black,hues,are,caused,by,accumulation,anthocyanins,while,their,absence,results,yellow,orange,green,fruits,In,Prunus,genes,determinants,peach,QTLs,plant,organ,traits,map,LG3,region,three,copies,copy,number,this,differs,respect,there,least,one,them,1a,correlating,skin,objective,study,was,determine,possible,role,natural,variability,develop,molecular,marker,assisted,selection,MAS,We,explored,within,using,long,sequences,obtained,previous,studies,through,CRISPR,Cas9,enrichment,sequencing,found,that,only,expressed,Its,promoting,biosynthesis,validated,transient,overexpression,analysis,identified,explained,individuals,analyzed,hypothesize,may,activate,pathway,propose,first,candidate,variation,provide
AB值：
0.401199
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