典型文献
Real-time tracking of ER turnover during ERLAD by a rhenium complex via lifetime imaging
文献摘要：
Endoplasmic reticulum(ER)degradation by autophagy(ER-phagy)is a recently revealed selective autophagy pathway that plays important roles in organelle turnover and protein degradation,but the biological functions of ER-phagy are largely unknown.Here,we present an ER-targeting Re(I)tricarbonyl complex(Re-ERLAD)that can accumulate in the ER,induce ER-to-lysosome-associated degradation(ERLAD)upon visible light irradiation,and label ER buds and track their morphological alterations during ER-phagy.The emission of Re-ERLAD is sensitive to viscosity,which is a key parameter reflecting the amount of unfolded protein in the ER.Quantitative detection using two-photon fluorescence lifetime imaging microscopy shows that ER viscosity initially increases and then decreases during ERLAD,which reveals that ERLAD is a pathway for alleviating ER stress caused by unfolded proteins.In conclusion,our work presents the first specific photoinducer and tracker of ERLAD,which can be used in studying the regulatory mechanism and function of this process.
文献关键词：
中图分类号：
作者姓名：
Liang Hao;Yu-Yi Ling;Zhi-Xin Huang;Zheng-Yin Pan;Cai-Ping Tan;Zong-Wan Mao
作者机构：
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry,School of Chemistry,State Key Laboratory of Oncology in South China,Sun Yat-Sen University,Guangzhou 510275,China
文献出处：
引用格式：
[1]Liang Hao;Yu-Yi Ling;Zhi-Xin Huang;Zheng-Yin Pan;Cai-Ping Tan;Zong-Wan Mao-.Real-time tracking of ER turnover during ERLAD by a rhenium complex via lifetime imaging)[J].国家科学评论（英文版）,2022(07):73-80
A类：
ERLAD,tricarbonyl,photoinducer
B类：
Real,tracking,turnover,during,by,rhenium,complex,lifetime,imaging,Endoplasmic,reticulum,degradation,autophagy,recently,revealed,selective,pathway,that,plays,important,roles,organelle,but,biological,functions,are,largely,unknown,Here,we,targeting,can,accumulate,lysosome,associated,upon,visible,light,irradiation,label,buds,their,morphological,alterations,emission,sensitive,viscosity,which,key,parameter,reflecting,amount,unfolded,Quantitative,detection,using,two,photon,fluorescence,microscopy,shows,initially,increases,then,decreases,reveals,alleviating,stress,caused,proteins,In,conclusion,our,work,presents,first,specific,tracker,studying,regulatory,mechanism,this,process
AB值：
0.517653
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
