典型文献
Dissection the endocytic routes of viral capsid proteins-coated upconversion nanoparticles by single-particle tracking
文献摘要：
Real-time exploring the cellular endocytic pathway of viral capsid proteins(VCPs)functionalized nanocar-gos at the single-particle level can provide deep insight into the kinetic information involved in virus in-fection.In this work,porcine circovirus type 2(PCV2)VCPs with different functions are modified onto the surface of upconversion nanoparticles(VCPs-UCNPs)to investigate the cellular internalization process in real-time.Clathrin-mediated endocytosis is found to be the essential uptake mechanism for these VCPs-UCNPs.Besides,it is verified that P1-UCNPs(PCV2 VCPs with nuclear localization signal,namely P1)can be easily assembled close to the perinuclear area,which is different from that of P2-UCNPs(PCV2 VCPs without nuclear localization signal,namely P2).Interestingly,multistep entry processes are observed.Par-ticularly,confined diffusion is observed during the transmembrane process.The intracellular transport of VCPs-UCNPs is dependent on microtubules toward the cell interior.During this process,P1-UCNPs dis-play increased velocities with active transport,while diffusion much faster around the perinuclear area.But for P2-UCNPs,there are only two phases involved in their endocytosis process.This study presents distinct dynamic mechanisms for the nanocargos with different functions,which would make a useful contribution to the development of robust drug delivery systems.
文献关键词：
中图分类号：
作者姓名：
Yujun Ning;Lin Wei;Shen Lin;Yifan Jiang;Naidong Wang;Lehui Xiao
作者机构：
College of Chemistry,Zhengzhou University,Zhengzhou 450001,China;State Key Laboratory of Medicinal Chemical Biology,Tianjin Key Laboratory of Biosensing and Molecular Recognition,College of Chemistry,Nankai University,Tianjin 300071,China;College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha 410081,China;College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;School of Chemistry and Chemical Engineering,Henan Normal University,Xinxiang 453007,China
文献出处：
引用格式：
[1]Yujun Ning;Lin Wei;Shen Lin;Yifan Jiang;Naidong Wang;Lehui Xiao-.Dissection the endocytic routes of viral capsid proteins-coated upconversion nanoparticles by single-particle tracking)[J].中国化学快报（英文版）,2022(10):4710-4714
A类：
endocytic,VCPs,nanocar,gos,Clathrin,nanocargos
B类：
Dissection,routes,viral,capsid,proteins,coated,upconversion,nanoparticles,by,single,tracking,Real,exploring,pathway,functionalized,level,can,provide,deep,insight,into,kinetic,information,involved,fection,this,work,porcine,circovirus,type,PCV2,different,functions,modified,onto,surface,UCNPs,investigate,internalization,real,mediated,endocytosis,found,be,essential,uptake,these,Besides,verified,that,P1,localization,signal,namely,easily,assembled,close,perinuclear,area,which,from,P2,without,Interestingly,multistep,entry,processes,observed,Par,ticularly,confined,diffusion,during,transmembrane,intracellular,transport,dependent,microtubules,toward,interior,During,play,increased,velocities,active,while,much,faster,around,But,there,only,two,phases,their,This,study,presents,distinct,dynamic,mechanisms,would,make,useful,contribution,development,robust,drug,delivery,systems
AB值：
0.515928
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