Serine/threonine phosphatase calcineurin (CN) is a unique but confounding calcium/calmodulin-mediated enzyme,which is composed of a catalytic subunit A (CNA) and a regulatory subunit B (CNB).We cloned six transcripts for CNA named from NICNA-X1 to NICNA-X6,one CNB named NICNB1 and one CNB homologous gene NICNBH1 from Nilaparvata lugens.All of them are constitutively transcripted in various tissues and developmental stages.The primary structure of the six isoforms showed obvious differences in the length and composition of the amino acid sequence between the two binding domains of Ca2+/calmodulin (CaM) and CNB.Ca2+-binding EF-hand motifs were found in NICNB1 and NICNBH1.The specific gene silencing of NICNA,NICNB1 and NICNBH1 respectively by RNAi resulted in drastical reduction in survival rate,female weight,eclosion rate and fecundity of N.lugens.These results showed that NICNA,NICNB1 and NICNBH1 were required for N.lugens growth and reproduction.The negative effects of NICNB1 silence on nymph mortality (97％),molting malformation (90％) and female sterile (50％)were more serious than those of NICNA or NICNBH1.qRT-PCR and enzyme-linked immunosorbent assay (ELISA) analyses indicated that the nymphs with silenced NICNA,NICNB1 or NICNBH1 showed impaired hormone and energy metabolism.In nymphs,the contents of 20-hydroxyecdysone (20E) after NICNB1 RNAi and phenoloxidase after NICNA RNAi were particularly decreased.These results suggested that NICNA is involved in immunity of N.lugens by regulation of phenoloxidase,while NICNB1 may control the growth and development of N.lugens by 20E signaling pathway in addition to interact with CNA.Injection of 70 ng/μL dsNICNB1 resulted in 77.0％ down regulation of NICNB1,and the nymph mortality was up to 57.9％ at 10 d after injection.Therefore,NICNB1 could be a potential candidate target used for strategy design in control of N.lugens.Our results revealed the importance of CN in the regulation of the growth and development of N.lugens,which provided a basis for further study of the molecular mechanism of CN.

WANG Weixia;ZHU Tingheng;WAN Pinjun;WEI Qi;HE Jiachun;LAI Fengxiang;FU Qiang

State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China;College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,China

水稻科学（英文版）

[1]WANG Weixia;ZHU Tingheng;WAN Pinjun;WEI Qi;HE Jiachun;LAI Fengxiang;FU Qiang-.Cloning and Functional Analysis of Calcineurin Subunits A and B in Development and Fecundity of Nilaparvata lugens (St(a)l))[J].水稻科学（英文版）,2022(02):143-154,中插434-中插438

Subunits,Fecundity,NICNA,NICNB1,NICNBH1,transcripted,drastical,phenoloxidase,dsNICNB1

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