首站-论文投稿智能助手
典型文献
Identification of the genetic central dogma in osteogenic differentiation of MSCs by osteoinductive medium from transcriptional data sets
文献摘要:
Background::The genetic central dogma (GCD) has been demonstrated its essential function in many biological processes and diseases. However, its roles in the process of osteogenic differentiation of mesenchymal stem cells (MSCs) remain unclear.Methods::In this project, we analyzed an online database of osteogenic differentiation of MSCs after 14 days and 28 days by osteoinductive medium (GSE83770). The differentially expressed genes were screened by GEO2R, with further conducting of KEGG pathways using DAVID. In addition, protein-protein interactions of the enriched pathways were performed using STRING with marked hub genes measured by the CytoHubba. Hub genes were verified by quantitative reverse-transcription polymerase chain reaction.Results::Results showed that six pathways related to GCD, including DNA replication, Aminoacyl-tRNA biosynthesis, Mismatch repair, Ribosome, Spliceosome, and RNA degradation pathways enriched in the early stage (14 days vs. undifferentiated MSCs) of osteogenesis. The Lysosome pathway was highly enriched in the late stage (28 vs. 14 days) of osteogenesis, and Ribosome pathway plays a key role throughout the entire process (28 days vs. undifferentiated MSCs) of osteogenesis.Conclusion::Both DNA replication and protein translation were functionally worked in the early stage of osteogenesis, whereas the Lysosome pathway was the only GCD-related one in the late stage of osteogenesis. The GCD-related Ribosome pathway occupied the entire process of osteogenesis.
文献关键词:
genetic central dogma;lysosome;mesenchymal stem cells;osteogenic differentiation;ribosome
作者姓名:
Jiang Tong-Meng
作者机构:
School of Materials Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China
引用格式:
[1]Jiang Tong-Meng-.Identification of the genetic central dogma in osteogenic differentiation of MSCs by osteoinductive medium from transcriptional data sets)[J].慢性疾病与转化医学(英文),2022(03):218-228
A类:
dogma,osteoinductive,GSE83770,Aminoacyl,Ribosome,Spliceosome
B类:
Identification,genetic,central,osteogenic,differentiation,MSCs,by,medium,from,transcriptional,sets,Background,GCD,has,been,demonstrated,its,essential,many,biological,processes,diseases,However,roles,mesenchymal,stem,cells,remain,unclear,Methods,In,this,project,analyzed,online,database,after,days,differentially,expressed,were,screened,GEO2R,further,conducting,pathways,using,DAVID,addition,protein,interactions,enriched,performed,STRING,marked,hub,measured,CytoHubba,verified,quantitative,reverse,polymerase,chain,reaction,Results,showed,that,six,related,including,replication,tRNA,biosynthesis,Mismatch,repair,degradation,early,stage,undifferentiated,osteogenesis,Lysosome,was,highly,plays,key,throughout,entire,Conclusion,Both,translation,functionally,worked,whereas,only,one,occupied,lysosome,ribosome
AB值:
0.445161
相似文献
Globular adiponectin-mediated vascular remodeling by affecting the secretion of adventitial-derived tumor necrosis factor-αinduced by urotensin Ⅱ
Jun LI;Limin LUO;Yonggang ZHANG;Xiao DONG;Shuyi DANG;Xiaogang GUO;Wenhui DING-Department of Cardiology,the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China;Division of Cardiology,Department of Internal Medicine,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China;Division of Cardiology,Department of Internal Medicine,Peking University First Hospital,Beijing 100034,China;Department of Dermatology,the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China;Department of Cardiovascular Diseases,the Second Affiliated Hospital,Shantou University Medical College,Shantou 515041,China
Identification of hub genes and their novel diagnostic and prognostic significance in pancreatic adenocarcinoma
Duo Zuo;Yongzi Chen;Xinwei Zhang;Zhuozhi Wang;Wenna Jiang;Fan Tang;Runfen Cheng;Yi Sun;Lu Sun;Li Ren;Rui Liu-Department of Clinical Laboratory;Department of Tumor Cell Biology,Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Tianjin's Clinical Research Center for Cancer,Tianjin 300060,China;School of Biomedical Engineering,Tianjin Medical University,Tianjin 300070,China;Department of Pathology,Tianjin Huanhu Hospital,Tianjin 300350,China;Department of Pathology,Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Tianjin's Clinical Research Center for Cancer,Tianjin 300060,China;School of Pharmacy,Tianjin Medical University,Tianjin 300070,China;Department of Gastrointestinal Medical Oncology,Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Tianjin's Clinical Research Center for Cancer,Tianjin 300060,China
Identification of circRNA/miRNA/mRNA regulatory net-work involving(+)-catechin ameliorates diabetic nephropathy mice
Chao Chen;Dina Zhu;Shuai Zhang;Wensheng Zhang-Zhuhai Branch of State Key Laboratory of Earth Surface Processes and Resource Ecology,Advanced Institute of Natural Sciences,Beijing Normal University,Zhuhai 519087,China;Engineering Research Center of Natural Medicine,Ministry of Education,Advanced Insti-tute of Natural Sciences,Beijing Normal University,Zhuhai 519087,China;International Cooperation Laboratory of Molecular Medicine,Academy of Chinese Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization,Faculty of Geographical Science,Beijing Normal University,Beijing 100875,China
机标中图分类号,由域田数据科技根据网络公开资料自动分析生成,仅供学习研究参考。