典型文献
ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines
文献摘要：
Objective: The aim of the present study was to investigate the mechanisms responsible for the radiation-sensitizing effect of antennapedia proteins, ANTP-SMACN7, on lung cancer cells treated with accelerated carbon and Fe particle irradiation.Methods: The ANTP-SMACN7 fusion peptide was synthesized and linked to fluorescein isothiocyanate to determine its ability to penetrate cells. A549 and NCI-H460 cells, human non-small cell lung cancer (NSCLC) cell lines, were irradiated with X-ray or high linear energy transfer (LET) irradiation with or without ANTP-SMACN7 treatment. Cellular survival, apoptosis, and protein expression were studied by colony formation assays, flow cytometry, and western blot analyses, respectively. Results: ANTP-SMACN7 fusion proteins entered the cells and promoted A549 and NCI-H460 cell high LET irradiation radiosensitization. High LET irradiation was more efficient for clonogenic cell killing and the induction of apoptosis (P < 0.05). Treatment with ANTP-SMACN7 significantly reduced the A549 and NCI-H460 cell clone-forming percentages and increased apoptosis through inhibition of the X-linked inhibitor of apoptosis protein and the activation of caspase-3 and caspase-9. Conclusions: Regarding pharmaceutical radiosensitization, these findings provided a way to improve high-LET clinical radiotherapy for NSCLC patients.
文献关键词：
中图分类号：
作者姓名：
Yi Xie;Bing Wang;Liqing Du;Yan Wang;Chang Xu;Hong Zhang;Kaixue Wen;Qiang Liu;Takanori Katsube
作者机构：
Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China;Advanced Energy Science and Technology Guangdong Laboratory,Huizhou 516029,China;National Institute of Radiological Sciences,National Institutes for Quantum and Radiological Science and Technology,Chiba 263-8555,Japan;Institute of Radiation Medicine,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300192,China;Shanxi Bethune Hospital,Shanxi Academy of Medical Science,Taiyuan 030031,China
文献出处：
引用格式：
[1]Yi Xie;Bing Wang;Liqing Du;Yan Wang;Chang Xu;Hong Zhang;Kaixue Wen;Qiang Liu;Takanori Katsube-.ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines)[J].癌症生物学与医学（英文版）,2022(07):983-994
A类：
ANTP,SMACN7,antennapedia,clonogenic
B类：
fusion,peptide,alone,induced,high,linear,energy,transfer,irradiation,radiosensitization,small,lung,cancer,lines,Objective,aim,present,study,was,investigate,mechanisms,responsible,sensitizing,effect,proteins,cells,treated,accelerated,carbon,particle,Methods,synthesized,linked,fluorescein,isothiocyanate,determine,its,ability,penetrate,A549,NCI,H460,human,NSCLC,were,irradiated,ray,LET,without,treatment,Cellular,survival,apoptosis,expression,studied,by,colony,formation,assays,flow,cytometry,western,blot,analyses,respectively,Results,entered,promoted,High,more,efficient,killing,induction,Treatment,significantly,reduced,clone,forming,percentages,increased,through,inhibition,inhibitor,activation,caspase,Conclusions,Regarding,pharmaceutical,these,findings,provided,way,improve,clinical,radiotherapy,patients
AB值：
0.48505
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