典型文献
Establishment of a pseudovirus neutralization assay based on SARS-CoV-2 S protein incorporated into lentiviral particles
文献摘要：
The coronavirus disease 2019 (COVID-19) is still causing a wide range of infections and deaths due to the high variability of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therefore, it is necessary to establish a reliable and convenient pseudovirus-based neutralization assay to develop drug targeted variants of SARS-CoV-2. Based on the HIV-1 backbone, we generated a high titer luciferase (Luc)-expressing pseudovirus packaging system. Three dominant S mutant substitution pseudovirus were also established and identified compared to wide type in hACE2-overexpressing HEK-293T cells (293T-ACE2 cells). Compared to serine protease inhibitor camostat mesylate, the cysteine protease inhibitor E-64d could significantly block all SARS-CoV-2 mutant S pseudovirus infection in 293T-ACE2 cells. Furthermore, the neutralization ability of two antibodies targeted receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) was evaluated, which showed different inhibition dose-effect curves among four types of S pseudovirus. Overall, we developed a pseudovirus-based neutralization assay for SARS-CoV-2, which would be readily adapted to SARS-CoV-2 variants for evaluating antibodies.
文献关键词：
COVID-19;SARS-CoV-2 variants;Pseudovirus;Neutralizing antibody;RBD
中图分类号：
作者姓名：
Wang Sheng;Liu Lizhen;Wang Can;Wang Ziqiang;Duan Xuhua;Chen Gang;Zhou Hu;Shao Hong
作者机构：
Department of Biochemical Drugs and Biological Products, Shanghai Institute for Food and Drug Control, Shanghai 201203, China;NMPA Key Laboratory for Quality Control of Therapeutic Monoclonal Antibodies, Shanghai Institute for Food and Drug Control, Shanghai 201203, China;School of Pharmacy, Fudan University, Shanghai 201203, China;Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China;Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
文献出处：
引用格式：
[1]Wang Sheng;Liu Lizhen;Wang Can;Wang Ziqiang;Duan Xuhua;Chen Gang;Zhou Hu;Shao Hong-.Establishment of a pseudovirus neutralization assay based on SARS-CoV-2 S protein incorporated into lentiviral particles)[J].生物安全与健康 （英文）,2022(01):38-44
A类：
camostat,Pseudovirus
B类：
Establishment,pseudovirus,neutralization,assay,SARS,CoV,protein,incorporated,into,lentiviral,particles,coronavirus,disease,still,causing,wide,range,infections,deaths,due,high,variability,severe,acute,respiratory,syndrome,Therefore,necessary,reliable,convenient,drug,targeted,variants,Based,HIV,backbone,generated,titer,luciferase,Luc,packaging,system,Three,dominant,mutant,substitution,were,also,established,identified,compared,hACE2,overexpressing,HEK,293T,cells,Compared,serine,protease,inhibitor,mesylate,cysteine,64d,could,significantly,block,Furthermore,two,antibodies,receptor,binding,domain,RBD,spike,was,evaluated,which,showed,different,inhibition,dose,effect,curves,among,four,types,Overall,developed,would,be,readily,adapted,evaluating,Neutralizing,antibody
AB值：
0.556673
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