OBJECTIVE:To explore biomarkers of Pien Tze Huang(片仔癀)that ameliorated the symptoms of hepatic fibrosis.METHODS:Two groups of carbon tetrachloride-induced hepatic fibrosis(HF)mice model were constructed in our study:one group received PZH treatment and another group received no treatment.We performed this study to investigate the role of PZH in the regulation process of hepatic fibrosis.RESULTS:We identified 31 down-regulated and 39 up-regulated miRNAs using small RNA-seq analysis.Combining RNA-Seq data analysis,our study revealed 7 significant target genes(Sp4,Slc2a6,Tln2,Hmga2,Ank3,Pax9,Fgf9).The results of real-time quantitative polymerase chain reaction analysis suggested that the expression level of 6 genes(Sp4,TIn2,Hmga2,Ank3,Pax9,Fgf9)were down-regulated compared to control group.On the other hand,the expression level of Slc2a6 appeared to be up-regulated.The protein mass spectrometry showed that PZH group had lower protein expression of Tln2 compared to control group.CONCLUSION:We identified 7 genes that were significantly related to PZH response in HF mice using multiple conjoint analysis methods.These genes could participate in underlying regulation mechanism of hepatic fibrosis during PZH treatment.

ZHANG Di;WEI Muyun;CHEN Luan;WU Hao;WANG Ting;ZHANG Zhiruo;ZHANG Ying;YU Juan;HUANG Jinming;ZHU Jinhang;QIN Shengying

School of Life Sciences,Anhui Medical University,Hefei 230032,China;Bio-X Institutes,Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders,Ministry of Education,Shanghai Jiao Tong University,Shanghai 200030,China;Fujian Provincial Key Laboratory of Pien Tze Huang Natural Medicine Research and Development,Zhangzhou Pien Tze Huang Pharmaceutical Co.,Ltd.,Zhangzhou 350000,China

中医杂志（英文版）

[1]ZHANG Di;WEI Muyun;CHEN Luan;WU Hao;WANG Ting;ZHANG Zhiruo;ZHANG Ying;YU Juan;HUANG Jinming;ZHU Jinhang;QIN Shengying-.Drug response biomarkers of Pien Tze Huang(片仔癀)treatment for hepatic fibrosis induced by carbon tetrachloride)[J].中医杂志（英文版）,2022(04):530-538

Pien,Tze,PZH,Sp4,Slc2a6,Tln2,Hmga2,Ank3,Pax9,Fgf9,TIn2

Drug,response,biomarkers,Huang,片仔癀,treatment,hepatic,fibrosis,induced,by,carbon,tetrachloride,OBJECTIVE,To,explore,that,ameliorated,symptoms,METHODS,Two,groups,HF,mice,model,were,constructed,our,study,one,received,another,We,performed,this,investigate,role,regulation,process,RESULTS,identified,down,regulated,miRNAs,using,small,seq,analysis,Combining,Seq,data,revealed,target,genes,results,real,quantitative,polymerase,chain,reaction,suggested,expression,level,compared,control,On,hand,appeared,be,protein,mass,spectrometry,showed,had,lower,CONCLUSION,significantly,related,multiple,conjoint,methods,These,could,participate,underlying,mechanism,during

0.409287