典型文献
Effect of aqueous extract of Astragalus membranaceus on behavioral cognition of rats living at high altitude
文献摘要：
OBJECTIVE:To investigate the effect of aqueous extract of Astragalus membranaceus on cognitive ability of rats living at high altitude.METHODS:Rats were exposed to a simulated high-altitude hypobaric hypoxia chamber.The behavior of rats was tested by eight-arm maze.The contents of malondialdehyde(MDA),glutathione(GSH),reactive oxygen species(ROS)and activity of total superoxide dismutase(T-SOD)in hippocampus were measured.The expressions of mammalian target of rapamycin(mTOR)and cleaved capase-3 in hippocampus were determined by reverse transcription-polymerase chain reaction and Western blot.RESULTS:The behavioral cognitive ability of the hypoxic control group was significantly lower than that of the normoxic control group.Under hypoxic environment,after the administration of aqueous extract of Astragalus membranaceus,the behavioral cognitive ability of rats was significantly improved.In hippocampal tissue,the content of MDA and ROS were significantly decreased,while the content of GSH and activity of T-SOD in hippocampus were significantly increased.The mRNA expression of mTOR and P70S6K and the protein expression of p-mTOR were significantly increased;the mRNA expression of 4E-binding protein 1(4E-BP1)and the protein expression of phosphorylated-4E-BP1(p-4E-BP1)and cleaved capase-3 were significantly decreased.CONCLUSION:When the rats are exposed to high altitude hypoxia,the behavioral cognitive ability could be significantly reduced.Aqueous extract of Astragalus membranaceus can significantly improve cognitive function in rats under hypoxia.The potential mechanism is related to improving oxidative stress,reducing the accumulation of free radicals and metabolites,and activating mTOR signaling pathway.
文献关键词：
中图分类号：
作者姓名：
DU Xing;LIU Tianlong;TAO Wendi;LI Maoxing;LI Xiaolin;YAN Lan
作者机构：
Gansu Institute for Drug Control,Lanzhou 730050,China;Department of Clinical Pharmacy,940th Hospital of Joint Logistic Support Force of PLA,Lanzhou 730050,China;Gansu Plateau Pharmaceutical Technology Center,Lanzhou 730050,China
文献出处：
引用格式：
[1]DU Xing;LIU Tianlong;TAO Wendi;LI Maoxing;LI Xiaolin;YAN Lan-.Effect of aqueous extract of Astragalus membranaceus on behavioral cognition of rats living at high altitude)[J].中医杂志（英文版）,2022(01):58-64
A类：
hypobaric
B类：
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AB值：
0.476543
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