典型文献
Efficient multinucleotide deletions using deaminase-Cas9 fusions in human cells
文献摘要：
CRISPR/Cas9 system is a robust genome editing platform in biotechnology and medicine.However,it generally produces small insertions/deletions(indels,typically 1-3 bp)but rarely induces larger deletions in specific target sites.Here,we report a cytidine deaminase-Cas9 fusion-induced deletion system(C-DEL)and an adenine deaminase-Cas9 fusion-induced deletion system(A-DEL)by combining Cas9 with rat APOBEC1(rA1)and TadA 8e,respectively.Both C-DEL and A-DEL improve the efficiency of deletions compared with the conventional Cas9 system in human cells.In addition,the C-DEL system generates a considerable fraction of predictable multinucleotide deletions from 5'-deaminated C bases to the Cas9-cleavage site and increases the proportion of larger deletions at the target loci.Taken together,the C-DEL and A-DEL systems provide a practical strategy for producing efficient multinucleotide deletions,expanding the CRISPR/Cas9 toolsets for gene modifications in human cells.
文献关键词：
中图分类号：
作者姓名：
Siyu Chen;Zhiquan Liu;Hao Yu;Liangxue Lai;Zhanjun Li
作者机构：
Key Laboratory of Zoonosis Research,Ministry of Education,College of Animal Science,Jilin University,Changchun,Jilin 130062,China;CAS Key Laboratory of Regenerative Biology,Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,South China Institute for Stem Cell Biology and Regenerative Medicine,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,Guangdong 510530,China;Guangzhou Regenerative Medicine and Health Guang Dong Laboratory(GRMH-GDL),Guangzhou,Guangdong 510005,China;Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,China
文献出处：
引用格式：
[1]Siyu Chen;Zhiquan Liu;Hao Yu;Liangxue Lai;Zhanjun Li-.Efficient multinucleotide deletions using deaminase-Cas9 fusions in human cells)[J].遗传学报,2022(10):927-933
A类：
multinucleotide,APOBEC1,rA1,TadA,deaminated,toolsets
B类：
Efficient,deletions,using,deaminase,Cas9,fusions,human,cells,CRISPR,is,robust,genome,editing,platform,biotechnology,medicine,However,generally,produces,small,insertions,indels,typically,bp,but,rarely,induces,larger,specific,target,sites,Here,report,cytidine,induced,DEL,adenine,by,combining,8e,respectively,Both,improve,efficiency,compared,conventional,In,addition,generates,considerable,fraction,predictable,from,bases,cleavage,increases,proportion,loci,Taken,together,systems,provide,practical,strategy,producing,efficient,expanding,modifications
AB值：
0.479677
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