A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosac-charides.Glycosyl acceptors terminated with a thiol tag were extended by enzymatic modular assembly(EMA)in the aqueous phase,and the desired products were captured during solid-phase workup(SPW)using commercially available thiopropyl Sepharose 6B resin through a reversible disulfide linkage.Finally,the products were released from solid-phase resin in the presence of dithio-threitol(DTT)as reducing agent and the resin could be recovered and reused.This strategy overcomes the uncertain influence of polymer-or ionic-supports commonly used in enzymatic glycosylation,and showed perfect compatibility with all 10 enzyme mod-ules for the rapid assembly of a series of complex oligosaccharides.

National Glycoengineering Research Center,NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine,and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology,Shandong University,Qingdao,Shandong 266237,China;Key Laboratory of Marine Drugs of Ministry of Education,Shandong Key Laboratory of Glycoscience and Glycotechnology,School of Medicine and Pharmacy,Ocean University of China,Qingdao,Shandong 266003,China;College of Pharmacy,Nankai University,Tianjin 300071,China;Glycobiology and Glycotechnology Research Center,College of Food Science and Technology,Northwest University,Xi'an,Shaanxi 710069,China;Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao,Shandong 266237,China

[1]Wenyuan Fang;Kan Zhong;Jiansong Cheng;Xian-Wei Liu;Chang-Cheng Liu;Zhongfu Wang;Hongzhi Cao-.Capture-Release Strategy Facilitates Rapid Enzymatic Assembly of Oligosaccharides)[J].中国化学（英文版）,2022(03):343-350

Oligosaccharides,oligosac,Glycosyl,thiopropyl,dithio,threitol

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