典型文献
Genomic and metabolomic analysis of Bacillus Ilchenlformls with enhanced poly-γ-glutamic acid production through atmospheric and room temperature plasma mutagenesis
文献摘要：
Poly-γ-glutamic acid is an extracellular polymeric substance with various applications owing to its valuable properties of biodegradability,flocculating activity,water solubility,and nontoxicity.However,the ability of natural strains to produce poly-γ-glutamic acid is low.Atmospheric and room temperature plasma was applied in this study to conduct mutation breeding of Bacillus licheniformis CGMCC 2876,and a mutant strain M32 with an 11％increase in poly-γ-glutamic acid was obtained.Genome resequencing analysis identified 7 nonsynonymous mutations of ppsC encoding lipopeptide synthetase associated with poly-γ-glutamic acid metabolic pathways.From molecular docking,more binding sites and higher binding energy were speculated between the mutated plipastatin synthase subunit C and glutamate,which might contribute to the higher poly-γ-glutamic acid production.Moreover,the metabolic mechanism analysis revealed that the upregulated amino acids of M32 provided substrates for glutamate and promoted the conversion between L-and D-glutamate acids.In addition,the glycol-ytic pathway is enhanced,leading to a better capacity for using glucose.The maximum poly-γ-glutamic acid yield of 14.08 g·L-1 was finally reached with 30 g·L-1 glutamate.
文献关键词：
中图分类号：
作者姓名：
Xiaoyu Wei;Lijie Yang;Haiyan Wang;Zhen Chen;Yiyuan Xu;Yue Weng;Mingfeng Cao;Qingbiao Li;Ning He
作者机构：
Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;College of Life Science,Xinyang Normal University,Xinyang 464000,China;College of Food and Biological Engineering,Jimei University,Xiamen 361021,China
文献出处：
引用格式：
[1]Xiaoyu Wei;Lijie Yang;Haiyan Wang;Zhen Chen;Yiyuan Xu;Yue Weng;Mingfeng Cao;Qingbiao Li;Ning He-.Genomic and metabolomic analysis of Bacillus Ilchenlformls with enhanced poly-γ-glutamic acid production through atmospheric and room temperature plasma mutagenesis)[J].化学科学与工程前沿,2022(12):1751-1760
A类：
Ilchenlformls,flocculating,ppsC,lipopeptide,plipastatin,ytic
B类：
Genomic,metabolomic,analysis,Bacillus,enhanced,glutamic,production,through,atmospheric,room,temperature,plasma,mutagenesis,Poly,extracellular,polymeric,substance,various,applications,owing,its,valuable,properties,biodegradability,activity,water,solubility,nontoxicity,However,natural,strains,produce,low,Atmospheric,was,applied,this,study,conduct,breeding,licheniformis,CGMCC,mutant,M32,increase,obtained,Genome,resequencing,identified,nonsynonymous,mutations,encoding,synthetase,associated,metabolic,pathways,From,molecular,docking,more,binding,sites,higher,energy,were,speculated,between,mutated,synthase,subunit,glutamate,which,might,contribute,Moreover,mechanism,revealed,that,upregulated,amino,acids,provided,substrates,promoted,conversion,In,addition,glycol,leading,better,capacity,using,glucose,maximum,yield,finally,reached
AB值：
0.577136
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