Nanoscale bismuth oxyiodide(nano-BiOI)is widely studied and applied in environmen-tal applications and biomedical fields,with the consequence that it may be deposited into aquatic environments.However,the impact of nano-BiOI on aquatic ecosystems,especially freshwater microalga,remains limited.Herein,the nano-BiOI was synthesized and its re-sponse mechanism towards microalga Chlamydomonas reinhardtii was evaluated.Results showed that a low concentration of nano-BiOI(5 mg/L)could stimulate algal growth at the early stage of stress.With the increase in concentration,the growth rate of algal cells was inhibited and showed a dose effect.Intracellular reactive oxygen species(ROS)were signif-icantly induced and accompanied by enhanced lipid peroxidation,decreased nonspecific esterase activity,and significantly upregulated glutathione S-transferase activity(GST)ac-tivity.Mineral nutrient metabolism analysis showed that nano-BiOI significantly interfered with the mineral nutrients of the algae.Non-targeted metabolomics identified 35 differ-ent metabolites(DEMs,22 upregulated,and 13 downregulated)under 100 mg/L BiOI stress.Metabolic pathway analysis demonstrated that a high concentration of nano-BiOI signifi-cantly induced metabolic pathways related to amino acid biosynthesis,lipid biosynthesis,and glutathione biosynthesis,and significantly inhibited the sterol biosynthesis pathway.This finding will contribute to understanding the toxicological mechanisms of nano-BiOI on C.reinhardtii.

School of Life Sciences,Southwest University of Science and Technology,Mianyang 621010,China;College of Environment and Resources,Southwest University of Science and Technology,Mianyang 621010,China;Laboratory of Solid Waste Treatment and Resource Recycle,Ministry of Education,Mianyang 621010,China;College of Chemical and Environment Science,Shaanxi University of Technology,Hanzhong 723000,China

[1]Yan Huang;Jinlong Lai;Yang Huang;Xuegang Luo;Xu Yang;Zewei Liu;Yue Duan;Chen Li-.Response mechanism of Chlamydomonas reinhardtii to nanoscale bismuth oxyiodide(nano-BiOI):Integrating analysis of mineral nutrient metabolism and metabolomics)[J].环境科学学报（英文版）,2022(11):13-24

reinhardtii,microalga

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