We investigated the effect of iron oxide nanoparticles(Fe3O4 NPs,～17 nm in size)on the phenotype and metabolite changes in hemp(Cannabis sativa L.),an annual crop distributed worldwide.Hemp clones were grown in hydroponic cultures with Fe3O4 NPs(50,100,200,or 500 mg/L)for four weeks.TEM and ICP-MS were used to determine Fe3O4 NPs uptake and translocation.LC-MS-based metabolomics was employed to explore the deep insight into the effect of Fe3O4 NPs on hemp plants.The results revealed that plant growth enhanced gradually with increasing concentrations of given NPs up to 200 mg/L,which improved the fresh weight and dry weight by 36.13％and 74.68％,respectively,compared to the control.Even at a high dose(500 mg/L),Fe3O4 NPs promoted plant growth,including increased biomass and tissue length.NPs significantly increased the iron and chlorophyll content in plant tissues Increased catalase activity and reduced hydrogen peroxide content in hemp leaves suggested that the Fe3O4 NPs activated the defense system.TEM showed that NPs were abundantly attached to the cell wall and dispersed throughout the root cells.Metabolomics revealed that Fe3O4 NPs induced metabolic reprogramming in hemp leaves,including the up-regulation of carbohydrates and organic acids,and down-regulation of antioxidants,especially tetrahydrocannabinol(THC).The significantly up-regulated metabolites,including peonidin and 2-hydroxycinnamic acid,could be involved in photosynthesis in hemp plants.These results demonstrate the potential of Fe3O4 NPs for promoting hemp growth and decreasing the THC content at low doses.

Canhui Deng;Qing Tang;Zemao Yang;Zhigang Dai;Chaohua Cheng;Ying Xu;Xiaojun Chen;Xiaoyu Zhang;Jianguang Su

Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410082,China

环境科学与工程前沿

[1]Canhui Deng;Qing Tang;Zemao Yang;Zhigang Dai;Chaohua Cheng;Ying Xu;Xiaojun Chen;Xiaoyu Zhang;Jianguang Su-.Effects of iron oxide nanoparticles on phenotype and metabolite changes in hemp clones(Cannabis sativa L.))[J].环境科学与工程前沿,2022(10):119-129

peonidin

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