MoS2 nanosheets (NSs) are novel 2D nanomaterials (NMs) with potential uses in many areas,and there-fore oral exposure route to MoS2 NSs is plausible.Currently,MoS2 NSs are considered as biocompatible NMs,but there is lacking of systemic investigations to study the interactions of MoS2 NSs with intestinal cells.In this study,we exposed the 3D Caco-2 spheroids to MoS2 NSs or MoS2 powders (denoted as MoS2-bulk),and investigated the potential adverse effects of MoS2-materials based on transcriptomics and lipidomics analysis.As expected,both MoS2 NSs and MoS2-bulk were dose-dependently internalized into 3D Caco-2 spheroids but did not induce cytotoxicity,membrane disruption or decrease of thiols.How-ever,the Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis indicated that nutrient absorption and metabolism was decreased.One of the most significantly decreased KEGG pathways is fat digestion and absorption (map04975),and Western blotting analysis further showed that fatty acid binding protein 1 and apolipoprotein A1,key proteins involved in fat digestion and absorp-tion,were down-regulated by MoS2 NSs or MoS2-bulk.In addition,BODIPY 493/503 staining suggested that exposure to MoS2 NSs and MoS2-bulk decreased lipid levels in the spheroids.However,lipidomics data indicated that MoS2 materials only decreased 8 lipid classes,including lysophosphatidylcholine,lysodimethylphosphatidylethanolamine,N-acylethanolamine,ceramide phosphoethanolamines,ganglio-sides,lysosphingomyelin and sulfatide,whereas most of the lipid classes were indeed increased.In addi-tion,MoS2 NSs was more potent to decrease the lipid classes compared with MoS2-bulk.Combined,the results from this study showed that MoS2 NSs and bulk materials were non-cytotoxic but altered lipid profiles in 3D Caco-2 spheroids.

Min Xie;Chaobo Huang;Yongqi Liang;Shuang Li;Liping Sheng;Yi Cao

National Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources,College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha 410081,China;College of Chemical Engineering,Nanjing Forestry University (NFU),Nanjing 210037,China;Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education,Laboratory of Biochemistry,College of Chemistry,Xiangtan University,Xiangtan 411105,China;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards,School of Public Health,University of South China,Hengyang 421001,China

中国化学快报（英文版）

[1]Min Xie;Chaobo Huang;Yongqi Liang;Shuang Li;Liping Sheng;Yi Cao-.MoS2 nanosheets and bulk materials altered lipid profiles in 3D Caco-2 spheroids)[J].中国化学快报（英文版）,2022(01):293-297

thiols,map04975,lysodimethylphosphatidylethanolamine,acylethanolamine,phosphoethanolamines,ganglio,lysosphingomyelin

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